Substrate specificities of wild- and mutated-type farnesyl diphosphate synthases with artificial substrate homologs

• Nagaki Masahiko

  Graduate School of Science and Technology, Hirosaki University

• Miyata Kazuhiko

  Graduate School of Science and Technology, Hirosaki University

• Kawakami Jun

  Graduate School of Science and Technology, Hirosaki University

• Miyamoto Ryo

  Graduate School of Science and Technology, Hirosaki University

• Maki Yuji

  Department of Material and Biological Chemistry, Faculty of Science, Yamagata University

• Sagami Hiroshi

  Institute of Multidisciplinary Research for Advanced Materials, Tohoku University

• Koyama Tanetoshi

  Institute of Multidisciplinary Research for Advanced Materials, Tohoku University

抄録

6,7-Epoxygeranyl diphosphate was examined for reactivity as an allylic substrate of wild and mutated farnesyl diphosphate (FPP) synthases of Bacillus stearothermophilus. 6,7-Epoxygeranyl diphosphate was accepted by wild and mutated (Y81R, Y81D, and Y81S) farnesyl diphosphate synthases as a substrate in reaction with isopentenyl diphosphate, yielding 10,11-epoxyfarnesyl and 14,15-epoxygeranylgeranyl diphosphates, respectively.

収録刊行物

• Transactions of the Materials Research Society of Japan

  Transactions of the Materials Research Society of Japan 34 (3), 533-536, 2009

  一般社団法人 日本MRS

キーワード

• wild and mutated type of farnesyl diphosphate synthase
• substrate specificity
• homologs of isopentenyl diphosphate
• epoxygeranyl diphosphate
• epoxyfarnesol

詳細情報

• CRID: 1390282680490363008
• NII論文ID: 130005004039
• DOI: 10.14723/tmrsj.34.533
• COI: 1:CAS:528:DC%2BD1MXhsFKktbzP
• ISSN: 21881650
• ISSN: 13823469
• Web Site: https://www.jstage.jst.go.jp/article/tmrsj/34/3/34_533/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可