Phospholipid Polymer-covered Magnetic Nanoparticles for Tracking Intracellular Molecular Reaction

• Machida Naoki

  Department of Materials Engineering, School of Engineering, The University of Tokyo

• Inoue Yuuki

  Department of Materials Engineering, School of Engineering, The University of Tokyo

• Ishihara Kazuhiko

  Department of Materials Engineering, School of Engineering, The University of Tokyo Department of Bioengineering, School of Engineering, The University of Tokyo

Abstract

The objective of this study is to manufacture novel magnetic polymer nanoparticles with phospholipid polymer brush layers and functional groups to regulate the interactions with intercellular biomolecules or cells. The combination of the magnetic polymer nanoparticles and polymerase chain reaction technique would lead to the understanding of intracellular molecular reactions. In this study, we designed the polymer nanoparticles possessing a remote controllability and biomolecules-binding ability. Remote controllability was accomplished by encapsulating magnetic nanoparticles in the polymer nanoparticles via miniemulsion polymerization of styrene with Fe₃O₄ nanoparticles. To prevent the non-specific interaction with intracellular biomolecules, poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) brush layer was constructed on the nanoparticles by surface-initiated atom radical polymerization. The magnetic polymer nanoparticles were specifically transferred into cells by immobilizing cell-penetrating peptide at the end of poly(MPC) chains. In particular, the applied external magnetic field enhanced cellular uptake.

Journal

• Transactions of the Materials Research Society of Japan

  Transactions of the Materials Research Society of Japan 39 (4), 427-430, 2014

  The Materials Research Society of Japan

Keywords

• 2-Methacryloyloxyethyl phosphorylcholine
• phospholipid polymer brush layer
• magnetic polymer nanoparticles
• click chemistry
• cell-penetrating peptide

Details

• CRID: 1390001205512109184
• NII Article ID: 130005004373
• DOI: 10.14723/tmrsj.39.427
• ISSN: 21881650
• ISSN: 13823469
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed