Chemoprotective effects of antioxidants against formaldehyde-induced toxicity in human keratinocytes

• KIM Min Ji

  Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Korea

• KIM Young Woo

  Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Korea

• HAN Eun Young

  Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Korea

• NAM Yoon A

  Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Korea

• CHUNG Bu Young

  Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Korea

• KYUNG Minji

  Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Korea

• BANG Du Yeon

  Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Korea

• LIM Seong Kwang

  Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Korea

• KWON Mi Jung

  Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Korea

• CHO Myung Chan

  Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Korea

• KIM Hyung Sik

  College of Pharmacy, Pusan National University, Korea

• LEE Byung-Mu

  Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Korea

抄録

Formaldehyde is a free radical generating carcinogen, and causes irritation and dermal allergies in humans. It has been widely used in industrial settings, hospitals, cosmetics and textiles. Human keratinocytes (HaCaT cells) were used for formaldehyde cytotoxicity and protective effects of antioxidants (α-tocopherol, N-acetylcysteine, tryptophan and quercetin) against oxidative stress induced by formaldehyde. HaCaT cells treated with formaldehyde (0, 5, 25, 50, 100 or 300 ppm) for 24 h showed that cell viability was dose-dependently decreased. In addition, total glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activities and intracellular formate were significantly decreased whereas malondialdehyde (MDA) was increased after formaldehyde exposure. To test protective effects of antioxidants, HaCaT cells were pretreated with 1, 10 or 100 μM antioxidants for 3 h and exposed to 274 ppm formaldehyde for 24 h. Antioxidants treatment increased cell viability, total GSH, SOD, CAT activities and intraculluar formate, but reduced cellular MDA production in HaCaT cells. In conclusion, antioxidants treatment might be beneficial in scavenging oxidative radicals produced by formaldehyde exposure in HaCaT cells.

収録刊行物

• 日本毒性学会学術年会

  日本毒性学会学術年会 39.2 (0), AP-125-, 2012

  日本毒性学会

詳細情報

• CRID: 1390282680523869440
• NII論文ID: 130005009061
• DOI: 10.14869/toxpt.39.2.0.ap-125.0
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可