Complement inhibition alleviates paraquat-induced acute lung injury

• WANG Han Bin

  Affiliated Hospital of Academy of Military Medical Sciences, China

抄録

The widely used herbicide, paraquat (PQ), is highly toxic and claims thousands of lives from both accidental and voluntary ingestion. The pathological mechanisms of PQpoisoning–induced acute lung injury (ALI) are not well understood, and the role of complement in PQ induced ALI has not been elucidated. We developed and characterized a mouse model of PQ-induced ALI and studied the role of complement in the pathogenesis of PQ poisoning. Intraperitoneal administration of PQ caused dose- and time-dependent lung damage and mortality, with associated inflammatory response. Within 24 hours of PQ-induced ALI, there was significantly increased expression of the complement proteins, C1q and C3 in the lung. Expression of the anaphylatoxin receptors, C3aR and C5aR, was also increased. Compared with wild-type mice, C3-deficient mice survived significantly longer and displayed significantly reduced lung inflammation and pathology after PQ treatment. Similar reductions in PQ-induced inflammation, pathology, and mortality were recorded in mice treated with the C3 inhibitors, CR2-Crry, and alternative pathway specific CR2-fH. A similar therapeutic effect was also observed by treatment with either C3a receptor antagonist or a blocking C5a receptor monoclonal antibody. Together, these studies indicate that PQ induced ALI is mediated through receptor signaling by the C3a and C5a complement activation products that are generated via the alternative complement pathway, and that complement inhibition may be an effective clinical intervention for post exposure treatment of PQ-induced ALI.

収録刊行物

• 日本毒性学会学術年会

  日本毒性学会学術年会 39.2 (0), AS1-2-, 2012

  日本毒性学会

詳細情報

• CRID: 1390001205546539520
• NII論文ID: 130005009266
• DOI: 10.14869/toxpt.39.2.0.as1-2.0
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可