Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody
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- Ohshima Shino
- Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
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- Mori Syuuya
- Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
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- Shigenari Atsuko
- Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
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- Miyamoto Asuka
- Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
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- Takasu Masaki
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
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- Imaeda Noriaki
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
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- Nunomura Satoshi
- Division of Molecular Cell Immunology, Nihon University Graduate School of Medical Science
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- Okayama Yoshimichi
- Division of Molecular Cell Immunology, Nihon University Graduate School of Medical Science
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- Tanaka Masafumi
- Division of Molecular Cell Immunology, Nihon University Graduate School of Medical Science
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- Kitagawa Hitoshi
- Division of Molecular Cell Immunology, Nihon University Graduate School of Medical Science
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- Kulski Jerzy K.
- Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine Centre for Forensic Science, The University of Western Australia
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- Inoko Hidetoshi
- Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
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- Ando Asako
- Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
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- Kametani Yoshie
- Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
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抄録
CD117 is a cytokine receptor expressed on the surface of hematopoietic stem cells with a likely role in cell survival, proliferation and differentiation. In order to study the differentiation activity of porcine CD117 hematopoietic cells in vitro and in vivo we prepared an anti-swine CD117 Mab (2A1) with high specificity for flow-cytometrical analysis. The 2A1 Mab did not recognize mouse or human mast cells suggesting that 2A1 is species-specific. Swine bone marrow (BM) CD117+ cells differentiated in vitro mainly into erythroid and monocyte lineages in the methylcellulose-based colony assay. When the swine BM CD117+ cells were transplanted in vivo into immunodeficient NOG (NOD/SCID/IL-2gc-null) mice, a significant amount of swine CD45+ leukocytes, including CD3 positive T cells, were developed in the mice. These results revealed that the swine BM CD117+ cells possess hematopoietic stem/progenitor activity and when monitored in immunodeficient mice or in vitro they can develop into lymphoid, erythroid, and myeloid cells efficiently with the new monoclonal antibody.
収録刊行物
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- BioScience Trends
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BioScience Trends 8 (6), 308-315, 2014
特定非営利活動法人 バイオ＆ソーシャル・サイエンス推進国際研究交流会