Susceptibility to proteases of anti-Tn-antigen MLS128 binding glycoproteins expressed in human colon cancer cells
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- Oura Fumie
- Department of Applied Biochemistry, Tokai University School of Engineering
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- Yajima Yukiko
- Department of Applied Biochemistry, Tokai University School of Engineering
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- Nakata Munehiro
- Department of Applied Biochemistry, Tokai University School of Engineering
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- Taniue Kenzui
- Department of Applied Biochemistry, Tokai University School of Engineering
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- Nakada *Hiroshi
- Department of Applied Biochemistry, Tokai University School of Engineering
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- Yamamoto Kazuo
- Department of Applied Biochemistry, Tokai University School of Engineering
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- Fujita-Yamaguchi Yoko
- Department of Applied Biochemistry, Tokai University School of Engineering
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抄録
Anti-Tn antigen MLS128 monoclonal antibody was produced two decades ago by immunizing mice with "cancerous antigens" derived from LS180 colon cancer cells. Previous studies demonstrated that MLS128 bound to 110 kDa glycoprotein (GP) in colon cancer cells, thereby inhibiting cell growth. Extensive attempts have been made towards understanding the inhibitory action of MLS128 on colon cancer cell growth and solving the primary structure of 110 kDa GP. Since limited proteolysis of 110 kDa GP was observed in microdomain fractions that had been kept frozen for several years, susceptibility of 110 kDa GP to trypsin and other proteases as well as N-glycosidase F has been investigated. Furthermore, 110 kDa GP expression was examined in colon cancer cells independently cultured in Akiyama laboratory. In summary, 110 kDa GP contains N-glycans. It does not contain inter-disulfide bonds but appears to have intra-disulfides. It must contain multiple cleavage sites for trypsin and thermolysin since these proteases digested 110 kDa GP to MLS128-undetectable small fragments. It seems to contain cleavage sites for cathepsin D which could cause limited digestion. LS180 cells derived from Akiyama laboratory produced a limited proteolysis product-like 75 kDa GP. This study provides a structural basis for developing cancer diagnostics and therapeutics.
収録刊行物
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- BioScience Trends
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BioScience Trends 9 (1), 49-55, 2015
特定非営利活動法人 バイオ＆ソーシャル・サイエンス推進国際研究交流会