Pycnogenol Supplementation Promotes Lipolysis via Activation of cAMP-Dependent PKA in ob/ob Mice and Primary-Cultured Adipocytes

• HO Jin-Nyoung

  Department of Medical Nutrition, Kyung Hee University

• KIM Ok-Kyung

  Department of Medical Nutrition, Kyung Hee University

• NAM Da-Eun

  Department of Medical Nutrition, Kyung Hee University

• JUN Woojin

  Department of Food and Nutrition, Chonnam National University

• LEE Jeongmin

  Department of Medical Nutrition, Kyung Hee University Research Institute of Medical Nutrition, Kyung Hee University

書誌事項

タイトル別名

• Pycnogenol Supplementation Promotes Lipolysis via Activation of cAMP-Dependent PKA in <i>ob/ob</i> Mice and Primary-Cultured Adipocytes

抄録

This study investigated the PKA-dependent inhibitory effect of pycnogenol (Pyc) on lipolysis using ob/ob mice and primary mouse adipocytes. Supplementation of Pyc at 30 mg/kg significantly reduced body weight gain and visceral fat mass. The serum and hepatic triglyceride (TG) and total cholesterol (TC) levels were reduced by Pyc supplementation, and high density lipoprotein (HDL)-cholesterol level significantly increased. In addition, hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) mRNA levels increased with Pyc supplementation in adipose tissue of ob/ob mice. The treatment of primary cultured adipocytes with Pyc at 100 μg/mL significantly increased glycerol release, cAMP level by reduction of phosphodiestersae-3B (PDE3B), and HSL levels, but decreased protein levels of perilipin A and fatty acid synthetase (FAS). The PKA inhibitor (H89) clearly blocked the cellular levels of perilipin A and HSL, suggesting that Pyc promotes lipolysis of adipocytes through activation of cAMP-dependent PKA, resulting in induction of HSL and reduction of perilipin A. Therefore, this study may elucidate the possible mechanism of Pyc, which is a candidate for weight loss through stimulation of lipolysis.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ 60 (6), 429-435, 2014

  一般財団法人 学会誌刊行センター

キーワード

• pycnogenol
• C57BL/6J <i>ob</i>/<i>ob</i> mouse
• primary adipocytes
• PKA pathway

詳細情報

• CRID: 1390282681300744704
• NII論文ID: 130005060913
• NII書誌ID: AA00703822
• DOI: 10.3177/jnsv.60.429
• ISSN: 18817742
• ISSN: 03014800
• NDL書誌ID: 026008040
• PubMed: 25866307
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026008040
• Web Site: https://search.jamas.or.jp/link/ui/2015261351
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可