Arabidopsis LAZY1 is a peripheral membrane protein of which the carboxy-terminal fragment potentially interacts with microtubules

DOI Web Site 被引用文献1件 参考文献22件
  • Sasaki Shu
    Biosystems Science Course, Graduate School of Life Science, Hokkaido University
  • Yamamoto Kotaro T.
    Biosystems Science Course, Graduate School of Life Science, Hokkaido University Faculty of Science, Hokkaido University

この論文をさがす

抄録

LAZY1 is a protein involved in gravity signaling of shoot gravitropism of rice, maize and Arabidopsis. Although the lazy1 mutants have been well-characterized, the function of the LAZY1 protein is still largely unknown. In this study, we used fluorescence microscopy to examine the subcellular localization of Arabidopsis LAZY1 (AtLAZY1) and its truncated proteins fused to GFP in tobacco leaves. We found that AtLAZY1 localizes to the plasma membrane through the C-terminal region, suggesting that the putative trans-membrane domain in the N-terminal half is not required for localization. Next, we took a biochemical approach to investigate the membrane association of AtLAZY1. Transiently expressed AtLAZY1 in transgenic Arabidopsis was fractionated in an insoluble fraction that contained membranous compartments. AtLAZY1 was solubilized by a non-ionic detergent or at a high pH condition, suggesting that AtLAZY1 is a peripheral membrane protein. We also found that when expressed in tobacco the C-terminal part of AtLAZY1 co-localized with microtubules. A microtubule binding assay showed that the C-terminal half of AtLAZY1, which localized to the plasma membrane, interacted with microtubules in vitro. These results suggest that AtLAZY1 may function with microtubules at the periphery of the plasma membrane in the gravity signaling process.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (22)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ