Bioconversion of Styrene to Poly(hydroxyalkanoate) (PHA) by the New Bacterial Strain Pseudomonas putida NBUS12

• Tan Giin-Yu Amy

  Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University

• Chen Chia-Lung

  Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University

• Ge Liya

  Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University

• Li Ling

  Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University

• Tan Swee Ngin

  Natural Sciences and Science Education Academic Group, Nanyang Technological University

• Wang Jing-Yuan

  Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University

書誌事項

タイトル別名

• Bioconversion of Styrene to Poly(hydroxyalkanoate) (PHA) by the New Bacterial Strain <i>Pseudomonas putida</i> NBUS12

抄録

Styrene is a toxic pollutant commonly found in waste effluents from plastic processing industries. We herein identified and characterized microorganisms for bioconversion of the organic eco-pollutant styrene into a valuable biopolymer medium-chain-length poly(hydroxyalkanoate) (mcl-PHA). Twelve newly-isolated styrene-degrading Pseudomonads were obtained and partial phaC genes were detected by PCR in these isolates. These isolates assimilated styrene to produce mcl-PHA, forming PHA contents between 0.05±0.00 and 23.10±3.25% cell dry mass (% CDM). The best-performing isolate was identified as Pseudomonas putida NBUS12. A genetic analysis of 16S rDNA and phaZ genes revealed P. putida NBUS12 as a genetically-distinct strain from existing phenotypically-similar bacterial strains. This bacterium achieved a final biomass of 1.28±0.10 g L⁻¹ and PHA content of 32.49±2.40% CDM. The extracted polymer was mainly comprised of 3-hydroxyhexanoate (C₆ ), 3-hydroxyoctanoate (C₈ ), 3-hydroxydecanoate (C₁₀ ), 3-hydroxydodecanoate (C₁₂ ), and 3-hydroxytetradecanoate (C₁₄ ) monomers at a ratio of 2:42:1257:17:1. These results collectively suggested that P. putida NBUS12 is a promising candidate for the biotechnological conversion of styrene into mcl-PHA.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 30 (1), 76-85, 2015

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• styrene
• eco-pollutants
• medium-chain-length poly(hydroxyalkanoate) (mcl-PHA)
• biopolyester
• <i>Pseudomonas putida</i> NBUS12

詳細情報

• CRID: 1390282679322945408
• NII論文ID: 130005061720
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me14138
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 026292539
• PubMed: 25740622
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026292539
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可