Insecticide-Degrading Burkholderia Symbionts of the Stinkbug Naturally Occupy Various Environments of Sugarcane Fields in a Southeast Island of Japan

• Tago Kanako

  Environmental Biofunction Division, National Institute for Agro-Environmental Sciences

• Okubo Takashi

  Environmental Biofunction Division, National Institute for Agro-Environmental Sciences

• Itoh Hideomi

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Hokkaido

• Kikuchi Yoshitomo

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Hokkaido

• Hori Tomoyuki

  Research Institute for Environmental Management Technology, AIST

• Sato Yuya

  Research Institute for Environmental Management Technology, AIST

• Nagayama Atsushi

  Okinawa Prefectural Agricultural Research Center

• Hayashi Kentaro

  Environmental Biofunction Division, National Institute for Agro-Environmental Sciences

• Ikeda Seishi

  Memuro Research Station, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization

• Hayatsu Masahito

  Environmental Biofunction Division, National Institute for Agro-Environmental Sciences

書誌事項

タイトル別名

• Insecticide-Degrading <i>Burkholderia</i> Symbionts of the Stinkbug Naturally Occupy Various Environments of Sugarcane Fields in a Southeast Island of Japan

抄録

The stinkbug Cavelerius saccharivorus, which harbors Burkholderia species capable of degrading the organophosphorus insecticide, fenitrothion, has been identified on a Japanese island in farmers’ sugarcane fields that have been exposed to fenitrothion. A clearer understanding of the ecology of the symbiotic fenitrothion degraders of Burkholderia species in a free-living environment is vital for advancing our knowledge on the establishment of degrader-stinkbug symbiosis. In the present study, we analyzed the composition and abundance of degraders in sugarcane fields on the island. Degraders were recovered from field samples without an enrichment culture procedure. Degrader densities in the furrow soil in fields varied due to differences in insecticide treatment histories. Over 99% of the 659 isolated degraders belonged to the genus Burkholderia. The strains related to the stinkbug symbiotic group predominated among the degraders, indicating a selection for this group in response to fenitrothion. Degraders were also isolated from sugarcane stems, leaves, and rhizosphere in fields that were continuously exposed to fenitrothion. Their density was lower in the plant sections than in the rhizosphere. A phylogenetic analysis of 16S rRNA gene sequences demonstrated that most of the degraders from the plants and rhizosphere clustered with the stinkbug symbiotic group, and some were identical to the midgut symbionts of C. saccharivorus collected from the same field. Our results confirmed that plants and the rhizosphere constituted environmental reservoirs for stinkbug symbiotic degraders. To the best of our knowledge, this is the first study to investigate the composition and abundance of the symbiotic fenitrothion degraders of Burkholderia species in farmers’ fields.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 30 (1), 29-36, 2015

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• <i>Burkholderia</i>
• organophosphorus insecticide degradation
• symbiotic gut bacteria
• <i>Cavelerius saccharivorus</i>
• sugarcane

詳細情報

• CRID: 1390001204346276608
• NII論文ID: 130005061723
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me14124
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 026292469
• PubMed: 25736865
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026292469
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可