Basic helix-loop-helix transcription factor DEC1 regulates the cisplatin-induced apoptotic pathway of human esophageal cancer cells
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- SEINO Hiroko
- Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine
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- WU Yunyan
- Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine
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- MOROHASHI Satoko
- Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine
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- KAWAMOTO Takeshi
- Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Science
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- FUJIMOTO Katsumi
- Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Science
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- KATO Yukio
- Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Science
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- TAKAI Yoshihiro
- Departments of Radiology and Radiation Oncology, Hirosaki University Graduate School of Medicine
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- KIJIMA Hiroshi
- Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine
書誌事項
- タイトル別名
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- <b>Basic helix-loop-helix transcription factor DEC1 regulates the cisplatin-induced apoptotic pathway of human esophageal cancer </b><b>cells </b>
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抄録
DEC1 [basic helix-loop-helix (BHLH) E40/Stra13/Sharp2] and DEC2 (BHLHE41/Sharp1) are BHLH transcription factors that are associated with the regulation of apoptosis, cell proliferation, and circadian rhythms, as well as malignancy in various cancers. However, the roles of DEC1 and DEC2 expression in esophageal cancer are poorly understood. In this study, we examined the roles of DEC1 and DEC2 in human esophageal cancer TE 5 and TE 10 cells that had been treated with cis-diamminedichloroplatinum (II) (cisplatin: CDDP). Expression of DEC1 and DEC2 was decreased with CDDP treatment in TE 5 cells; however, knockdown or overexpression of DEC1/DEC2 had little effects on CDDP-induced apoptosis in TE 5 cells. DEC1 expression was up-regulated in CDDP-treated TE 10 cells, whereas DEC2 expression was unchanged. DEC1 knockdown by siRNA in TE 10 decreased the amount of cleaved poly (ADP-ribose) polymerase (PARP) after treatment with CDDP, whereas DEC2 knockdown had no effects on the amount of cleaved PARP in both the presence and absence of CDDP. We also demonstrated that DEC1 overexpression promoted cleaved PARP expression, whereas DEC2 overexpression had no effects on the amount of cleaved PARP in TE 10 cells. These results suggested that DEC1 has pro-apoptotic effects on human esophageal cancer TE 10 cells of well-differentiated type.
収録刊行物
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- Biomedical Research
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Biomedical Research 36 (2), 89-96, 2015
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