Localization of Rod Bipolar Cells in the Mammalian Retina Using an Antibody Against the α<sub>1</sub>c L-type Ca<sup>2+</sup> Channel
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- Huh Yu-Jin
- Department of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University
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- Choi Jae-Sik
- Department of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University
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- Jeon Chang-Jin
- Department of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University
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Bipolar cells transmit stimuli via graded changes in membrane potential and neurotransmitter release is modulated by Ca2+ influx through L-type Ca2+ channels. The purpose of this study was to determine whether the α1c subunit of L-type voltage-gated Ca2+ channel (α1c L-type Ca2+ channel) colocalizes with protein kinase C alpha (PKC-α), which labels rod bipolar cells. Retinal whole mounts and vertical sections from mouse, hamster, rabbit, and dog were immunolabeled with antibodies against PKC-α and α1c L-type Ca2+ channel, using fluorescein isothiocyanate (FITC) and Cy5 as visualizing agents. PKC-α-immunoreactive cells were morphologically identical to rod bipolar cells as previously reported. Their cell bodies were located within the inner nuclear layer, dendritic processes branched into the outer plexiform layer, and axons extended into the inner plexiform layer. Immunostaining showed that α1c L-type Ca2+ channel colocalized with PKC-α in rod bipolar cells. The identical expression of PKC-α and α1c L-type Ca2+ channel indicates that the α1c L-type Ca2+ channel has a specific role in rod bipolar cells, and the antibody against the α1c L-type Ca2+ channel may be a useful marker for studying the distribution of rod bipolar cells in mouse, hamster, rabbit, and dog retinas.
収録刊行物
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- Acta Histochemica et Cytochemica
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Acta Histochemica et Cytochemica 48 (2), 47-52, 2015
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詳細情報 詳細情報について
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- CRID
- 1390282679837968512
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- NII論文ID
- 130005068297
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- ISSN
- 13475800
- 00445991
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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- 使用不可