Establishment of a novel dwarf rat strain: cartilage calcification insufficient (CCI) rats

DOI Web Site Web Site PubMed 参考文献26件 オープンアクセス
  • TANAKA Masami
    Department of Food and Nutrition, Junior College Division, The University of Aizu, Aizu-Wakamatsu, Fukushima 965-8570, Japan
  • WATANABE Minoru
    Institute for Animal Experimentation, St. Marianna University Graduate School of Medicine, Kawasaki, Kanagawa 216-8511, Japan
  • YOKOMI Izuru
    Department of Pharmacology, St. Marianna University School of Medicine, Kawasaki, Kanagawa 216-8511, Japan
  • MATSUMOTO Naoki
    Department of Pharmacology, St. Marianna University School of Medicine, Kawasaki, Kanagawa 216-8511, Japan
  • SUDO Katsuko
    Animal Research Center, Tokyo Medical University, Tokyo 160-8402, Japan
  • SATOH Hitoshi
    Laboratory of Tumor Cell Biology, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo 108-8639, Japan
  • IGARASHI Tsuneo
    Animal Care Co., Ltd., Tokyo 164-0001, Japan
  • SEKI Azusa
    Hamri Co., Ltd. Tokyo110-0005, Japan
  • AMANO Hitoshi
    Department of Pharmacology, Osaka Dental University, Osaka 573-1121, Japan
  • OHURA Kiyoshi
    Department of Pharmacology, Osaka Dental University, Osaka 573-1121, Japan
  • RYU Kakei
    Department of Pharmacology, Showa University School of Medicine, Tokyo 142-8555, Japan
  • SHIBATA Shunichi
    Maxillofacial Anatomy, Department of Maxillofacial Biology, Maxillofacial/Neck Reconstruction, Graduate School, Tokyo Medical and Dental University, Tokyo 113-8549, Japan
  • NAGAYAMA Motohiko
    Department of Oral Pathology, Asahi University School of Dentistry, Hozumi, Gifu 501-0296, Japan
  • TANUMA Jun-ichi
    Department of Oral Pathology, Asahi University School of Dentistry, Hozumi, Gifu 501-0296, Japan

この論文をさがす

抄録

Rats with dwarfism accompanied by skeletal abnormalities, such as shortness of the limbs, tail, and body (dwarf rats), emerged in a Jcl-derived Sprague-Dawley rat colony maintained at the Institute for Animal Experimentation, St. Marianna University Graduate School of Medicine. Since the dwarfism was assumed to be due to a genetic mutation based on its frequency, we bred the dwarf rats and investigated their characteristics in order to identify the causative factors of their phenotypes and whether they could be used as a human disease model. One male and female that produced dwarf progeny were selected, and reproduction was initiated by mating the pair. The incidence of dwarfism was 25.8% among the resultant litter, and dwarfism occurred in both genders, suggesting that it was inherited in an autosomal recessive manner. At 12 weeks of age, the body weights of the male and female dwarf rats were 40% and 57% of those of the normal rats, respectively. In soft X-ray radiographic and histological examinations, shortening and hypoplasia of the long bones, such as the tibia and femur, were observed, which were suggestive of endochondral ossification abnormalities. An immunohistochemical examination detected an aggrecan synthesis disorder, which might have led to delayed calcification and increased growth plate thickening in the dwarf rats. We hypothesized that the principal characteristics of the dwarf rats were systemically induced by insufficient cartilage calcification in their long bones; thus, we named them cartilage calcification insufficient (CCI) rats.

収録刊行物

  • Experimental Animals

    Experimental Animals 64 (2), 121-128, 2015

    公益社団法人 日本実験動物学会

参考文献 (26)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ