Thermal and Pasting Properties, Morphology of Starch Granules, and Crystallinity of Endosperm Starch in the Rice SSI and SSIIIa Double-Mutant
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- Hayashi Mari
- Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University
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- Kodama Momoko
- Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University
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- Nakamura Yasunori
- Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University
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- Fujita Naoko
- Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University
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Starch is glucose polymer linked by α-1,4 and α-1,6 glucosidic bonds. The balance of activity between starch synthases (SSs) and branching enzymes (BEs) is important for formation of amylopectin structure. SSI and SSIIIa isozymes account for 60 and 30%, respectively, of soluble SS activity in developing rice endosperm. Complete loss of both SSI and SSIIIa activities induces sterility. By contrast, a double mutant (ss1L/ss3a) generated by crossing the leaky ss1 mutant, whose SS activity is approximately ca. 16% of the wild type, and the ss3a null mutant is fertile. Although there is only a significant residual SS activity in the developing endosperm of ss1L/ss3a, the yield and growth of the double-mutant line is sufficient. We analyzed the amylopectin chain-length distribution, thermal and pasting properties, morphologies of starch granules and amyloplasts, and crystallinity of endosperm starch in ss1L/ss3a and the parental mutant lines. The chain-length distribution pattern of ss1L/ss3a was similar to that of ss3a and there was an additive effect of the reduction of SSI activity on the chain-length of amylopectin in the ss3a background. These changes in the amylopectin chain-length distribution were considered to increase the gelatinization temperature of the starch. The gelatinized starch viscosity of the double-mutant and ss3a lines was very low. Most ss1L/ss3a starch granules were round-shaped, and the starch granular size was smaller than those of the parental mutant lines and the wild type. The starch crystallinity of ss1L/ss3a was slightly reduced compared with that of the wild type.
収録刊行物
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 62 (2), 81-86, 2015
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390001206292204544
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- NII論文ID
- 130005070809
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- NII書誌ID
- AA11809133
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- ISSN
- 18807291
- 13447882
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- NDL書誌ID
- 026405203
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可