Two Fructan 1-Exohydrolase Isoforms Hydrolyze Fructans in Edible Burdock (Arctium lappa L.) during Storage at a Low Temperature

• Ueno Keiji

  Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University

• Yokoshima Satoru

  Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University

• Sasajima Yuki

  Department of Food Sciences, Faculty of Dairy Science, Rakuno Gakuen University

• Ishiguro Yojiro

  Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University

• Yoshida Midori

  NARO Hokkaido National Agricultural Research Center

• Shiomi Norio

  Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University

• Onodera Shuichi

  Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University

書誌事項

タイトル別名

• Two Fructan 1-Exohydrolase Isoforms Hydrolyze Fructans in Edible Burdock (<i>Arctium lappa</i> L.) during Storage at a Low Temperature

抄録

Edible burdock (Arctium lappa L.) accumulates an inulin-type fructan. Inulin-type fructan in plant has been hydrolyzed by fructan 1-exohydrolases (1-FEH). We have previously reported on the cloning of aleh1, which encodes a 1-FEH in edible burdock. Here, we describe the cloning of aleh2, which encodes a 1-FEH isozyme in edible burdock, and the functional analysis of the recombinant protein of aleh2 (rAlEH2) that displays properties different from the aleh1 recombinant protein. A cDNA, named aleh2, was obtained by the RACE. The rAlEH2, which was produced by Pichia pastoris, showed 1-FEH activity. Unlike the recombinant protein of aleh1, the rAlEH2 is a 1-FEH enzyme that efficiently hydrolyzes longer-chain fructans than 1-kestose, such as nystose, fructosylnystose and inulin. The expression study in burdock revealed the induction of aleh1 and aleh2 genes by low temperature. These findings indicated that two 1-FEH isoforms were involved in the degradation of the fructan in burdock roots during low-temperature storage.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ 62 (2), 65-72, 2015

  日本応用糖質科学会

キーワード

• <i>Arctium lappa</i> L.
• burdock
• fructan 1-exohydrolase
• fructan
• low temperature

詳細情報

• CRID: 1390282681268921344
• NII論文ID: 130005070814
• NII書誌ID: AA11809133
• DOI: 10.5458/jag.jag.jag-2014_012
• ISSN: 18807291
• ISSN: 13447882
• NDL書誌ID: 026405169
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026405169
• Web Site: https://www.jstage.jst.go.jp/article/jag/62/2/62_jag.JAG-2014_012/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可