乳腺細胞診検体における CK5/14+p63+CK7/18 カクテル抗体による多重染色の基礎的検討

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  • Optimization of multiple staining with a CK5/14+p63+CK7/18 cocktail antibody for breast cytology specimens

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The aim of the present study was to obtain the optimal immunocytochemical staining condition for a CK5/14+p63+CK7/18 cocktail antibody.<br>Fine needle cytology smear slides from surgically resected breast nodules were used. Immunocytochemical slides were compared under four different antigen retrieval conditions.<br>Twenty minutes with pH9 retrieval produced good staining for the CK5/14+p63+CK7/18 cocktail antibody.<br>Only a few slides are available for breast cytology in routine practice. Multicolor immunocytochemistry using a cocktail antibody is becoming an important ancillary method.

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