Extraction of Apigenin and Luteolin from Leaves of Alfalfa (Medicago sativa L.) Using Mixtures of Liquid Carbon Dioxide and Ethanol

DOI Web Site 参考文献7件
  • MISHIMA Kenji
    Department of Chemical Engineering, Faculty of Engineering, Fukuoka University
  • KAWAMURA Hirofumi
    Department of Chemical Engineering, Faculty of Engineering, Fukuoka University Department of Seasoning & Foods Division, San-Ei Gen F.F.I.
  • KAWAKAMI Ryo
    Department of Chemical Engineering, Faculty of Engineering, Fukuoka University
  • ITO Shota
    Department of Chemical Engineering, Faculty of Engineering, Fukuoka University
  • INOUE Yuuta
    Department of Chemical Engineering, Faculty of Engineering, Fukuoka University
  • HIROTA Tetsuya
    Department of Chemical Engineering, Faculty of Engineering, Fukuoka University
  • HARADA Takunori
    Department of Chemical Engineering, Faculty of Engineering, Fukuoka University
  • KATO Takafumi
    Department of Chemical Engineering, Faculty of Engineering, Fukuoka University
  • SHARMIN Tanjina
    Department of Chemical Engineering, Faculty of Engineering, Fukuoka University
  • MISUMI Makoto
    Department of Electronics Engineering and Computer Science, Faculty of Engineering, Fukuoka University
  • SUETSUGU Tadashi
    Department of Electronics Engineering and Computer Science, Faculty of Engineering, Fukuoka University
  • IRIE Keiichi
    Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University
  • MISHIMA Kenichi
    Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University
  • MUSTOFA Salim
    Research Center for Technology of Nuclear Industrial Material, Indonesia Nuclear Energy Agency
  • HASANAH Fauziyah
    Faculty of Science and Technology, Syarif Hidayatullah State Islamic University (UIN) Jakarta
  • SIREGAR Yusraini Dian Inayati
    Faculty of Science and Technology, Syarif Hidayatullah State Islamic University (UIN) Jakarta
  • ZAHARO Hilyatuz
    Faculty of Science and Technology, Syarif Hidayatullah State Islamic University (UIN) Jakarta
  • PUTRI Lily Surayya Eka
    Faculty of Science and Technology, Syarif Hidayatullah State Islamic University (UIN) Jakarta
  • SALIM Agus
    Faculty of Science and Technology, Syarif Hidayatullah State Islamic University (UIN) Jakarta

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  • Extraction of Apigenin and Luteolin from Leaves of Alfalfa (<i>Medicago sativa</i> L.) Using Mixtures of Liquid Carbon Dioxide and Ethanol

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The extraction of apigenin and luteolin from the alfalfa leaves; Medicago sativa (L.) using mixtures of liquid carbon dioxide (CO2) was performed at 5, 20 and 25 ºC. The extraction pressure was from 8.5 to 14 MPa. The extraction yields were compared with yields obtained by other solvent extraction methods, such as pure liquid CO2 extraction and conventional alcohol (methanol or ethanol) extraction. We conducted qualitative and quantitative analyses for luteolin and apigenin in the extract using HPLC and analyzed the extraction behavior. The effect of three operating parameters, temperature, pressure and composition of ethanol in the liquid CO2 solution, on the extraction yield was investigated using the single-factor method. The yields of luteolin and apigenin in the extraction were significantly improved by adjusting the operating temperature and amount of ethanol in the liquid CO2 solution, whereas a change in the selectivity of the extract was not observed.

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