Gamma Ray and Ethyl Methane Sulphonate Induced Translocation and Inversion Heterozygote in <i>Lens culinaris </i>Medik (Lentil)

• Goyal Sapna

  School of Studies in Botany, Vikram University

• Verma Rakesh Chandra

  School of Studies in Botany, Vikram University

抄録

Seeds of Lens culinaris Medik (Lentil) were irradiated with various doses of gamma rays, (viz. 5 kR, 10 kR, 15 kR, 20 kR, 25 kR and 30 kR) and some seeds were also treated with different concentrations (0.1, 0.2, 0.3, and 0.4%) of Ethyl Methane Sulphonate (EMS) for 6 and 12 h in separate experiments. Two plants, one translocation heterozygote and one inversion heterozygote, were isolated from the population raised from 10 kR gamma ray-irradiated seeds and 0.2% EMS-treated seeds, respectively. The mutant plants displayed various types of chromosomal configurations at diakinesis/metaphase I, and anaphase/telophase I/II in meiosis. The translocation heterozygote exhibited the formation of ring/chain of four and six chromosomes in a majority of the PMCs at diakinesis/metaphase-I, and the inversion heterozygote was characterized by the presence of bridge and fragments at anaphase/telophase I/II because of various numbers and positions of crossovers in the inversion loop. Pollen fertility declined to 38% in the translocation heterozygote and 27.33% in the inversion heterozygote as compared to 96% in the control.

収録刊行物

• ＣＹＴＯＬＯＧＩＡ

  ＣＹＴＯＬＯＧＩＡ 80 (2), 231-235, 2015

  公益財団法人 日本メンデル協会

キーワード

• <i>Lens culinaris</i> (Lentil)
• Gamma ray
• EMS
• Translocation heterozygote
• Inversion heterozygote
• Pollen fertility

詳細情報

• CRID: 1390282679085686272
• NII論文ID: 130005074301
• DOI: 10.1508/cytologia.80.231
• ISSN: 13487019
• ISSN: 00114545
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可