<b>Orexin-A Inhibits the High Potassium-Induced Increase in Intracellular Calcium Levels in Cultured Dorsal Root Ganglions Neurons from Carrageenan-Treated Rats </b>

• Kimura Masataka

  Nihon University Graduate School of Dentistry at Matsudo, Removable Prosthodontics, Matsudo, Chiba 271-8587, Japan

• Ishikawa Manabu

  Nihon University Graduate School of Dentistry at Matsudo, Anesthesiology, Matsudo, Chiba 271-8587, Japan

• Suzuki Asako

  Nihon University Graduate School of Dentistry at Matsudo, Anesthesiology, Matsudo, Chiba 271-8587, Japan

• Uda Akio

  Departments of Anesthesiology, Nihon University School of Dentistry at Matsudo, Matsudo,Chiba 271-8587, Japan

• Okubo Masakazu

  Departments of Removable Prosthodontics, Nihon University School of Dentistry at Matsudo, Matsudo,Chiba 271-8587, Japan

• Iijima Morio

  Departments of Removable Prosthodontics, Nihon University School of Dentistry at Matsudo, Matsudo,Chiba 271-8587, Japan

• Kuboyama Noboru

  Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

• Kawai Yasuhiko

  Departments of Removable Prosthodontics, Nihon University School of Dentistry at Matsudo, Matsudo,Chiba 271-8587, Japan

抄録

We elucidated the effects of orexin-A in dorsal root ganglion (DRG) neurons. Immunohistochemical staining was performed to compare changes in N-methyl-daspartate receptors (NMDARs) and orexin-A in the DRG between the carrageenaninduced neuropathic pain rat model (CA) and non-algetic rats (Control). We also evaluated the inhibitory effects of orexin-A by measuring [Ca²⁺] i. Using immunohistochemistry, orexin-A expression was observed in both the posterior and anterior horns of the spinal cord. No major difference was observed between the groups. Using immunohistochemistry, orexin-A expression was also observed in neurons in the DRG, and no major difference was observed between the groups. NMDAR expression levels in the posterior horn of the spinal cord and the DRG were not quantitatively determined, but the overall staining intensity of NMDAR was higher in CA than in control rats. Also, in the DRG neurons of the CA rats, administration of orexin-A significantly suppressed the increase in [Ca²⁺] i due to depolarization stimulation caused by high K⁺. Thus, the anti-nociceptive effect was due to an increase in orexin-A. Furthermore, orexin-A may have specifically acted on L-type calcium channels.

収録刊行物

• ＩＪＯＭＳ

  ＩＪＯＭＳ 13 (3), 94-101, 2015

  日本大学松戸歯学部 口腔科学研究所

キーワード

• dorsal root ganglion (DRG), intracellular free calcium ions ([Ca²⁺]i), orexin-A, carrageenan-induced inflammation hyperalgesia

詳細情報

• CRID: 1390282680188808960
• NII論文ID: 130005076111
• DOI: 10.5466/ijoms.13.94
• ISSN: 21854254
• ISSN: 13479733
• Web Site: https://www.jstage.jst.go.jp/article/ijoms/13/3/13_94/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016061414
• 本文言語コード: en
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