Silica nanoparticle-induced toxicity in mouse lung and liver imaged by electron microscopy

• Isoda Katsuhiro

  Graduate School of Pharmaceutical Sciences, Teikyo Heisei University Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University

• Kondoh Masuo

  Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University

• Yoshioka Yasuo

  Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University

• Tsutsumi Yasuo

  Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University

• Imazawa Takayoshi

  Bioresources Research, Laboratory of Common Apparatus, National Institute of Biomedical Innovation

• Nishimura Tetsuji

  Graduate School of Pharmaceutical Sciences, Teikyo Heisei University

• Ishida Isao

  Graduate School of Pharmaceutical Sciences, Teikyo Heisei University

• Yagi Kiyohito

  Laboratory of Bio-Functional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Osaka University

抄録

Nanomaterials have been proposed as novel substrates for medical and commercial applications. However, such materials also may have novel toxicities, thus posing environmental and health concerns. We previously reported hepatic injury in mice following the intravenous administration of unmodified silica particles with diameters of 70 nm (SP70); this toxicity was not observed following administration by the same route of micro-size particles with diameters of 300 nm (SP300) or 1,000 nm (SP1000). In the present study, we used electron microscopy to investigate the dynamics of silica nanoparticles administered in mice. SP70 was observed in hepatocytes and in lung epithelial cells. Inclusion within hepatocytes was associated with accumulation of SP70 in the liver sinusoidal endothelial cells and passage through the space of Disse. In contrast, SP300 and SP1000 were not observed within the hepatocytes. To our knowledge, our report represents the first demonstration that silica nanoparticles accumulate in hepatocytes, liver sinusoidal endothelial cells, Kupffer cells, and lung tissue; accumulation of SP70 in liver sinusoidal endothelial cells correlated with the induction of liver injury.

収録刊行物

• Fundamental Toxicological Sciences

  Fundamental Toxicological Sciences 2 (1), 19-23, 2015

  一般社団法人 日本毒性学会

キーワード

• Silica nanoparticles
• Electron microscope
• Hepatocytes
• Liver sinusoidal endothelial cells
• Kupffer cells
• Lungs

詳細情報

• CRID: 1390001205762954752
• NII論文ID: 130005083355
• DOI: 10.2131/fts.2.19
• ISSN: 2189115X
• Web Site: https://www.jstage.jst.go.jp/article/fts/2/1/2_19/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016128349
• 本文言語コード: en
• データソース種別:
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