Hertwig’s Epithelial Root Sheath Fate during Initial Cellular Cementogenesis in Rat Molars

• Yamamoto Tsuneyuki

  Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine

• Yamada Tamaki

  Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine

• Yamamoto Tomomaya

  Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine

• Hasegawa Tomoka

  Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine

• Hongo Hiromi

  Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine

• Oda Kimimitsu

  Division of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences

• Amizuka Norio

  Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine

抄録

To elucidate the fate of the epithelial root sheath during initial cellular cementogenesis, we examined developing maxillary first molars of rats by immunohistochemistry for keratin, vimentin, and tissue non-specific alkaline phosphatase (TNALP) and by TdT-mediated dUTP nick end labeling (TUNEL). The advancing root end was divided into three sections, which follow three distinct stages of initial cellular cementogenesis: section 1, where the epithelial sheath is intact; section 2, where the epithelial sheath becomes fragmented; and section 3, where initial cellular cementogenesis begins. After fragmentation of the epithelial sheath, many keratin-positive epithelial sheath cells were embedded in the rapidly growing cellular cementum. A few unembedded epithelial cells located on the cementum surface. Dental follicle cells, precementoblasts, and cementoblasts showed immunoreactivity for vimentin and TNALP. In all three sections, there were virtually no cells possessing double immunoreactivity for vimentin-keratin or TNALP-keratin and only embedded epithelial cells showed TUNEL reactivity. Taken together, these findings suggest that: (1) epithelial sheath cells divide into two groups; one group is embedded in the cementum and thereafter dies by apoptosis, and the other survives on the cementum surface as epithelial cell rests of Malassez; and (2) epithelial sheath cells do not undergo epithelial-mesenchymal transition during initial cellular cementogenesis.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 48 (3), 95-101, 2015

  日本組織細胞化学会

キーワード

• Hertwig’s epithelial root sheath
• cementoblasts
• epithelial-mesenchymal transition
• cellular cementum
• rat molars

詳細情報

• CRID: 1390001204862776832
• NII論文ID: 130005083853
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.15006
• ISSN: 13475800
• ISSN: 00445991
• Web Site: https://www.jstage.jst.go.jp/article/ahc/48/3/48_15006/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016216108
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
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