Increased expression of 5-HT2A and 5-HT2B receptors in detrusor muscle after partial bladder outlet obstruction in rats

MICHISHITA Mai, YANO Kazuo, KASAHARA Ken-ichi, TOMITA Ken-ichi, MATSUZAKI Osamu

doi:10.2220/biomedres.36.187

Serotonin (5-hydroxytryptamine; 5-HT)-induced bladder contraction is enhanced after partial bladder outlet obstruction (pBOO) in rats. We investigated time-dependent changes in bladder contraction and expression of 5-HT_2A and 5-HT_2B receptor mRNA in bladder tissue to elucidate the mechanism of this enhancement. On day 3 and 7 after pBOO, contractile responses of isolated rat bladder strips to 5-HT were increased compared with that in sham-operated rats; on day 14, the response had decreased to the same level as that in sham rat bladders. In contrast, carbacholinduced contraction was not enhanced by pBOO at any time point. In sham rats, 5-HT_2A receptor mRNA was expressed in the urothelium, and 5-HT_2B receptor mRNA was expressed in the detrusor muscle layer. In pBOO rats, both receptor mRNAs were increased in the detrusor muscle and subserosal layers, but not in the urothelium. The increase of 5-HT_2A receptor mRNA was maintained from day 3 to day 14 after pBOO, and 5-HT_2B receptor mRNA was increased on day 7 after pBOO. These results suggested that pBOO induced up-regulation of the 5-HT_2A and 5-HT_2B receptors in the detrusor muscle and subserosal layers of the bladder, and such up-regulation may be related to the enhanced bladder contractile response to 5-HT.

<b>Increased expression of 5-HT</b><b><sub>2A</sub></b><b> and 5-HT</b><b><sub>2B</sub></b><b> receptors in detrusor muscle after partial bladder outlet obstruction in </b><b>rats </b>

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