Quality Evaluation and Pattern Recognition Analyses of Bioactive Marker Compounds from Farfarae Flos Using HPLC/PDA
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- Seo U Min
- College of Pharmacy, Catholic University of Daegu
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- Zhao Bing Tian
- College of Pharmacy, Catholic University of Daegu
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- Kim Won Il
- College of Pharmacy, Catholic University of Daegu
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- Seo Eun Kyoung
- College of Pharmacy and Division of Life and Pharmaceutical Sciences, Ewha Womans University
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- Lee Jae Hyun
- College of Oriental Medicine, Dongguk University
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- Min Byung Sun
- College of Pharmacy, Catholic University of Daegu
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- Shin Beom Soo
- College of Pharmacy, Catholic University of Daegu
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- Son Jong Keun
- College of Pharmacy, Yeungnam University
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- Woo Mi Hee
- College of Pharmacy, Catholic University of Daegu
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抄録
The flower bud of Tussilago farfara L., called Farfarae Flos, has traditionally been used in Oriental medicine for the treatment of bronchitis and asthma. To establish a standard for quality control as well as the reliable identification of Farfarae Flos, the contents of five standards, rutin (1), isoquercetin (2), 3,5-dicaffeoylquinic acid (3), tussilagone (4), and tussilagonone (5), were determined by quantitative high-performance liquid chromatography (HPLC)/photodiode array (PDA) analysis. The five standards were separated on a YoungJinBioChrom Aegispak C18-L (250-mm×4.6-mm, 5-µm) column by gradient elution using 0.03% trifluoroacetic acid in water (A), with acetonitrile (B) as the mobile phase. The flow rate was 1.0 mL/min, and the UV detector wavelength was set at 220 nm. The method was successfully used in the analysis of Farfarae Flos from different geographic origins with relatively simple conditions and procedures, and the results demonstrated satisfactory linearity, recovery, precision, accuracy, stability, and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 62 Farfarae Flos samples. This result indicated that the established HPLC/PDA method is suitable for quantitation and pattern recognition analyses for the quality evaluation of Farfarae Flos.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 63 (7), 546-553, 2015
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679153859456
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- NII論文ID
- 130005085942
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- NII書誌ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- NDL書誌ID
- 026549201
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- PubMed
- 25971744
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可