Agrobacterium-mediated transformation and regeneration of Freesia×hybrida

• Uwagaki Yohei

  Ishikawa Agriculture and Forestry Research Center Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

• Matsuda Eriko

  Ishikawa Agriculture and Forestry Research Center

• Komaki Masako

  Ishikawa Agriculture and Forestry Research Center

• Murahama Minoru

  Ishikawa Agriculture and Forestry Research Center

• Otani Motoyasu

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

• Nishizawa Naoko

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

• Hamada Tatsuro

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

書誌事項

タイトル別名

• <i>Agrobacterium</i>-mediated transformation and regeneration of <i>Freesia</i>×<i>hybrida</i>

抄録

A method for Agrobacterium-mediated transformation of Freesia×hybrida is described. Cormlet-derived calli of two cultivars, ‘Mosera’ and ‘Ishikawa f3’ were co-cultivated with Agrobacterium tumefaciens strain EHA105 harboring the binary vector pIG121-Hm, which included hygromycin phosphotransferase gene and an intron-containing β-glucuronidase gene in the T-DNA region. Callus pieces were co-cultivated with A. tumefaciens on the callus proliferation medium [Murashige and Skoog (MS) medium containing 1 mg l⁻¹ thidiazuron, 1 mg l⁻¹ dicamba, 20 mg l⁻¹ 3′,5′-dimethoxy-4′-hydroxyacetophenone, 1% (w/v) glucose, 3% (w/v) sucrose, and 0.2% (w/v) Gelrite]. Then, they were cultured on the callus proliferation medium containing 300 mg l⁻¹ cefatoxime and 10 mg l⁻¹ hygromycin B. Hygromycin-resistant lines of both cultivars regenerated into plantlets after transfer onto MS medium containing 2 mg l⁻¹ 3-indoleacetic acid and 3 mg l⁻¹ 6-benzyl aminopurine and/or plant growth regulator-free MS medium. Transgenic plants were identified by β-glucuronidase assay and verified by Southern blot analysis. Two transgenic plant lines were obtained from 475 callus pieces of ‘Mosera’, and one transgenic plant line was obtained from 290 callus pieces of ‘Ishikawa f3’. This is the first report of the genetic transformation of Freesia. This method will allow the genetic improvement of this horticulturally important flower.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 32 (2), 165-168, 2015

  日本植物バイオテクノロジー学会

キーワード

• <i>Agrobacterium tumefaciens</i>
• cormlet
• <i>Freesia</i>×<i>hybrida</i>
• genetic transformation
• nodular callus

詳細情報

• CRID: 1390282679304219776
• NII論文ID: 130005085990
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.15.0301a
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 026560201
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026560201
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可