Serum Metabolomics in Rats after Acute Paraquat Poisoning

• Wang Zhiyi

  The Second Affiliated Hospital & Yuying Children’s Hospital, Wenzhou Medical University

• Ma Jianshe

  Function Experiment Teaching Center, Wenzhou Medical University

• Zhang Meiling

  Analytical and Testing Center, Wenzhou Medical University

• Wen Congcong

  Analytical and Testing Center, Wenzhou Medical University

• Huang Xueli

  Analytical and Testing Center, Wenzhou Medical University

• Sun Fa

  Analytical and Testing Center, Wenzhou Medical University

• Wang Shuanghu

  The Laboratory of Clinical Pharmacy, People’s Hospital of Lishui City

• Hu Lufeng

  Department of Clinical Pharmacy, The First Affiliated Hospital of Wenzhou Medical University

• Lin Guanyang

  Department of Clinical Pharmacy, The First Affiliated Hospital of Wenzhou Medical University

• Wang Xianqin

  Analytical and Testing Center, Wenzhou Medical University

抄録

Paraquat is one of the most widely used herbicides in the world and is highly toxic to humans and animals. In this study, we developed a serum metabolomic method based on GC/MS to evaluate the effects of acute paraquat poisoning on rats. Pattern recognition analysis, including both principal component analysis and partial least squares-discriminate analysis revealed that acute paraquat poisoning induced metabolic perturbations. Compared with the control group, the level of octadecanoic acid, L-serine, L-threonine, L-valine, and glycerol in the acute paraquat poisoning group (36 mg/kg) increased, while the levels of hexadecanoic acid, D-galactose, and decanoic acid decreased. These findings provide an overview of systematic responses to paraquat exposure and metabolomic insight into the toxicological mechanism of paraquat. Our results indicate that metabolomic methods based on GC/MS may be useful to elucidate the mechanism of acute paraquat poisoning through the exploration of biomarkers.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 38 (7), 1049-1053, 2015

  公益社団法人 日本薬学会

キーワード

• metabolomics
• GC/MS
• paraquat
• poisoning
• rat

詳細情報

• CRID: 1390282679610268416
• NII論文ID: 130005086291
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b15-00147
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 026548954
• PubMed: 26133715
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026548954
• Web Site: https://www.jstage.jst.go.jp/article/bpb/38/7/38_b15-00147/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016091168
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可