MC3T3-E1 Cell Assay on Collagen or Fibronectin Immobilized Poly (Lactic Acid-ε-Caprolactone) Film

DOI Web Site 被引用文献2件 参考文献22件
  • Fuse Megumi
    Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo
  • Hayakawa Tohru
    Department of Dental Engineering, Tsurumi University School of Dental Medicine
  • Hashizume-Takizawa Tomomi
    Department of Oral Immunology, Nihon University School of Dentistry at Matsudo
  • Takeuchi Reiri
    Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
  • Kurita-Ochiai Tomoko
    Department of Oral Immunology, Nihon University School of Dentistry at Matsudo
  • Fujita-Yoshigaki Junko
    Department of Physiology, Nihon University School of Dentistry at Matsudo
  • Fukumoto Masahiko
    Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo

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In the present study, collagen (Coll) or fibronectin (FN) was immobilized onto poly(lactic acid)/ε-caprolactone (PLCL) copolymer surface and the attachment and proliferation of osteoblast-like cells on Coll or FN immobilized PLCL (Coll-PLCL, FN-PLCL) were evaluated. Coll or FN immobilized PLCL was prepared through a condensation reaction between the carboxylic acid groups on the hydrolyzed PLCL (PLCL-COOH) surface and the amino groups of Coll or FN using water soluble carbodiimide. The contact angle of the PLCL-COOH, Coll-PLCL and FN-PLCL surfaces with respect to double distilled water significantly decreased compared with PLCL (p<0.05). Number of attached cells onto Coll-PLCL or FN-PLCL films was significantly greater than on PLCL films from at the time of 90 min to 7days (p<0.05). The morphological differences of attached osteoblast-like cells were observed between PLCL films and Coll-PLCL or FN-PLCL films at the early stage of cell assay. The cell attached on Coll-PLCL and FN-PLCL films appeared more flat than on PLCL films. Additionally, many actin filaments and stress fibers were observed on Coll-PLCL or FN-PLCL films during 3 and 7 days of cell assay. Actin filaments and stress fibers were few on PLCL films. These data demonstrated the effectiveness of collagen and fibronectin immobilization of PLCL for cell attachment and proliferation. This indicates that collagen or fibronectin immobilization of PLCL films was effective for biological activities of osteoblast-like cells.

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