Controlled release of simvastatin from biodegradable hydrogels promotes odontoblastic differentiation
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- MIYAZAWA Atsuko
- Department of Oral & Maxillofacial Surgery, The Nippon Dental University School of Life Dentistry Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University
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- MATSUNO Tomonori
- Department of Oral & Maxillofacial Surgery, The Nippon Dental University School of Life Dentistry
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- ASANO Kazunari
- Department of Oral & Maxillofacial Surgery, The Nippon Dental University School of Life Dentistry
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- TABATA Yasuhiko
- Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University
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- SATOH Tazuko
- Department of Oral & Maxillofacial Surgery, The Nippon Dental University School of Life Dentistry
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抄録
The objective of this study was to investigate the odontoblastic differentiation of dental pulp stem cells (DPSC) by biodegradable hydrogels incorporating simvastatin micelles, both in vitro and in vivo. Simvastatin (ST) was incorporated into the micelles of gelatin grafted with L-lactic acid oligomers (LAo) to allow water-solubilization. The simvastatin-LAo-grafted gelatin (LAo-g-gelatin) micelles were mixed with gelatin, followed by chemical crosslinking to form gelatin hydrogels (ST Mi/GH). The ST Mi were released from the gelatin hydrogel granules (GH) through enzymatic degradation. The ST Mi enhanced alkaline phosphatase activity, calcium deposition, and bone morphogenic protein-2 secretion of DPSC. When implanted subcutaneously into mice, the ST Mi/GH treated group exhibited increased dentin sialoprotein and calcium deposition, compared with those treated with GH plus free ST. It is possible to achieve odontoblastic differentiation of DPSC through the controlled release of ST from GH.
収録刊行物
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- Dental Materials Journal
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Dental Materials Journal 34 (4), 466-474, 2015
日本歯科理工学会