Using Acetate and Formate as the Substrates for Geobacter sulfurreducens Exoelectrogenesis Resulted in Different Half-saturation Potentials

• PENG Luo

  School of Resources & Environment, Southwest University Biofunctional Catalysts Research Team, RIKEN Center for Sustainable Resource Science

• ZHANG Xiao-Ting

  School of Resources & Environment, Southwest University

• KAWAICHI Satoshi

  Biofunctional Catalysts Research Team, RIKEN Center for Sustainable Resource Science

• XIE De-Ti

  School of Resources & Environment, Southwest University

• LI Zhen-Lun

  School of Resources & Environment, Southwest University

書誌事項

タイトル別名

• Using Acetate and Formate as the Substrates for <i>Geobacter sulfurreducens</i> Exoelectrogenesis Resulted in Different Half-saturation Potentials

抄録

In bioelectrochemical systems where the oxidative current is mediated by microorganisms, it remains unexplored as to whether low-potential substrates (e.g. formate) enable the anode to work at lower potentials. Due to implications to relevant engineering and natural systems, this study evaluated such possibility and underlying causes. The investigation compared voltammograms of the model exoelectrogen (to exclude the interfering factors in undefined cultures) Geobacter sulfurreducens grown with acetate and formate. G. sulfurreducens had an E_M (half-saturation potential) of −0.138 ± 0.004 V vs. SHE when consuming acetate; an E_M of −0.160 ± 0.002 V when utilizing formate. Such variation usually requires alternation in electrode reductase expressed by bacteria, according to the existing Nernst-Monod model with a single species of electron conduit. For both acetate- and formate-grown biofilm, non-catalytic voltammetries found multiple redox couples with distinct formal potentials. No clear evidence could support a hypothesis that the bacteria synthesized any different electron conduits when the substrate was changed. Significant changes in the relative abundance of high-potential and low-potential electrocatalytically active conduits were not observed as well. However, low-potential conduits showed elevated electrocatalytic activities in the formate-grown biofilm, which might induce the shift in apparent E_M.

収録刊行物

• Electrochemistry

  Electrochemistry 83 (8), 600-604, 2015

  公益社団法人　電気化学会

キーワード

• <i>Geobacter sulfurreducens</i>
• Extracellular Electron Transfer
• Microbial Fuel Cell
• Half-Saturation Potential

詳細情報

• CRID: 1390282681475084288
• NII論文ID: 130005092001
• NII書誌ID: AN00151637
• DOI: 10.5796/electrochemistry.83.600
• ISSN: 21862451
• ISSN: 13443542
• NDL書誌ID: 026629901
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026629901
• Web Site: https://www.jstage.jst.go.jp/article/electrochemistry/83/8/83_14-E00099/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用可