Evaluation of Recanalization of Occluded Middle Cerebral Artery and Restored Cerebral Blood Flow in Rats with Transient Brain Ischemia: A Combination Study of Digital Subtraction Angiography and <sup>15</sup>O-water Positron Emission Tomography

DOI Web Site 被引用文献1件 参考文献25件
  • Watabe Tadashi
    Department of Molecular Imaging in Medicine PET Molecular Imaging Center
  • Kanai Yasukazu
    Department of Molecular Imaging in Medicine PET Molecular Imaging Center
  • Ikeda Hayato
    Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine
  • Matsunaga Keiko
    Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine Immunology Frontier Research Center, Osaka University
  • Kamiya Takashi
    Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine Division of Radiology, Department of Medical Technology
  • Kato Hiroki
    PET Molecular Imaging Center Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine
  • Horitsugi Genki
    Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine
  • Naka Sadahiro
    Department of Pharmacy, Osaka University Hospital
  • Isohashi Kayako
    PET Molecular Imaging Center Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine
  • Shimosegawa Eku
    Department of Molecular Imaging in Medicine PET Molecular Imaging Center Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine
  • Hatazawa Jun
    PET Molecular Imaging Center Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine Immunology Frontier Research Center, Osaka University

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The purpose of this study was to validate the usefulness of digital subtraction angiography (DSA) using the fluoroscopic mode of a small-animal PET/CT in detecting recanalization of occluded middle cerebral artery (MCA), and to evaluate reperfusion by 15O-water positron emission tomography (PET) in rats with transient unilateral occlusion of MCA. Methods: Rats with (n=6) and without transient unilateral MCA occlusion (n=6) were examined. DSA was performed in the X-ray fluoroscopic mode with small-animal PET/CT. An iodine contrast medium (0.3 mL) was administered by bolus injection via the catheter in the internal carotid artery under isoflurane anesthesia. A 10-min dynamic PET scanning was started simultaneously with an intravenous bolus injection of 15O-water during MCA occlusion (occlusion duration: 45 min) and approximately 30 min after recanalization in ischemic rats. Cerebral blood flow (CBF) was evaluated semi-quantitatively as CBF index determined from the 15O-water PET images, which is normalized to the injected dose per body weight. Results: DSA revealed recanalization of the ipsilateral MCA in the ischemic rat models. The 15O-water PET images showed decreased CBF during MCA occlusion and recovery of the CBF after recanalization in the ipsilateral MCA territory. The CBF index of the ipsilateral and contralateral MCA territories during MCA occlusion were 0.84±0.12 and 1.66±0.13, respectively, and those after recanalization were 1.24±0.17 and 1.67±0.10, respectively. The CBF index of the ipsilateral MCA territory increased significantly after recanalization (p=0.04), although it remained significantly lower than the CBF index of the contralateral MCA territory (p=0.04). Conclusions: This study demonstrated the feasibility of DSA using the X-ray fluoroscopic mode of a small-animal PET/CT. Recovery of the CBF was not sufficient at 30 min after recanalization in rats with transient MCA occlusion.

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