Expression of Vasohibin-1 in Human Carotid Atherosclerotic Plaque

• Fukumitsu Ryu

  Department of Neurosurgery, Kyoto University Graduate School of Medicine

• Minami Manabu

  Department of Clinical Innovative Medicine, Kyoto University Graduate School of Medicine

• Yoshida Kazumichi

  Department of Neurosurgery, Kyoto University Graduate School of Medicine

• Nagata Manabu

  Department of Neurosurgery, Kyoto University Graduate School of Medicine

• Yasui Mika

  Department of Clinical Innovative Medicine, Kyoto University Graduate School of Medicine

• Higuchi Sei

  Department of Clinical Innovative Medicine, Kyoto University Graduate School of Medicine

• Fujikawa Risako

  Department of Clinical Innovative Medicine, Kyoto University Graduate School of Medicine

• Ikedo Taichi

  Department of Neurosurgery, Kyoto University Graduate School of Medicine

• Yamagata Sen

  Department of Neurosurgery, Kurashiki Central Hospital

• Sato Yasufumi

  Department of Vascular Biology, Institute of Development, Aging, and Cancer, Tohoku University

• Arai Hidenori

  Department of Human Health and Sciences, Kyoto University Graduate School of Medicine

• Yokode Masayuki

  Department of Clinical Innovative Medicine, Kyoto University Graduate School of Medicine

• Miyamoto Susumu

  Department of Neurosurgery, Kyoto University Graduate School of Medicine

抄録

Aim: In patients with carotid plaque, intraplaque hemorrhage arising from ruptured neovascular vessels within the neointima is an important cause of stroke. The expression of Vasohibin-1 (VASH1), a negative feedback regulator of angiogenesis, occurs in the microvessel endothelial cells of various solid tumors and the arterial wall. However, the roles of VASH1 in the pathogenesis of atherosclerotic diseases remain unclear. The present study aimed to clarify the relevance of the VASH1 expression and plaque instability in human carotid plaques.<br>Methods: We used quantitative real-time PCR and immunostaining to examine 12 atheromatous plaque specimens obtained via carotid endarterectomy. The distal areas of specimens lacking macroscopic atherosclerotic lesions served as controls.<br>Results: Compared with that observed in the controls, the VASH1 gene expression increased significantly in the atheromatous plaque (p=0.018). Moreover, the VASH1 mRNA levels correlated positively with those of VEGFA, CD31 and VCAM1 (r=0.788, p=0.004; r=0.99, p＜0.001; r=0.94, p＜0.001, respectively). Finally, the immunohistochemical analyses revealed the VASH1 expression in the neointimal microvessel endothelial cells of carotid plaque.<br>Conclusions: The VASH1 expression levels in atheroma reflect both enhanced neovascularization and the inflammatory burden. Therefore, the VASH1 level may be a novel biomarker for evaluating plaque instability in patients with carotid arteriosclerosis and predicting ischemic stroke.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ　ａｎｄ　Ｔｈｒｏｍｂｏｓｉｓ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ　ａｎｄ　Ｔｈｒｏｍｂｏｓｉｓ 22 (9), 942-948, 2015

  一般社団法人 日本動脈硬化学会

キーワード

• Vasohibin-1
• Atherosclerosis
• Carotid artery
• Vulnerable plaque
• Neovascularization

詳細情報

• CRID: 1390282679410085120
• NII論文ID: 130005099293
• NII論文ID: 120006305929
• DOI: 10.5551/jat.29074
• ISSN: 18803873
• ISSN: 13403478
• HANDLE: 2433/225062
• PubMed: 25843115
• Web Site: https://www.jstage.jst.go.jp/article/jat/22/9/22_29074/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016247180
• 本文言語コード: en
• データソース種別:
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  • IRDB
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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