Microdroplets as a Model System for the Study of Macromolecular Crowding in Cells

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  • YANAGISAWA Miho
    Department of Applied Physics, Faculty of Engineering, Tokyo University of Agriculture and Technology
  • FUJIWARA Kei
    Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University

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  • 高分子混雑効果を細胞モデル系から読み解く
  • コウブンシ コンザツ コウカ オ サイボウ モデルケイ カラ ヨミ トク

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Abstract

Molecular crowding and confinement in small spaces are remarkable features of living cells. To reveal the physicochemical aspects of these features, the effects of confinement-size and droplet surface on them should be separated from them. Water-in-oil microdroplets covered with a lipid layer (droplet system) have suitable characters to separate them, because the droplet sizes and lipid species covering the droplet are easily controlled. Here, we introduce recent achievements using the droplet system that has revealed effects of confinements on protein expression, accelerated phase-separation of biopolymer blends, and behaviors of highly concentrated biopolymers.

Journal

  • Seibutsu Butsuri

    Seibutsu Butsuri 55 (5), 246-249, 2015

    The Biophysical Society of Japan General Incorporated Association

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