Thrombolytic and Antiplatelet Effects of a Novel Plasminogen Activator from the Venom of <i>Gloydius Brevicaudus</i> Viper
-
- Zhang Zhiqiang
- School of Pharmacy, Fujian Medical University
-
- Lin Lili
- School of Pharmacy, Fujian Medical University
-
- Zhang Jinhua
- Department of Pharmacy, Fujian Medical University Union Hospital
-
- Zhang Qiong
- School of Pharmacy, Fujian Medical University
-
- Zhao Ningning
- School of Pharmacy, Fujian Medical University
-
- Wu Linqun
- School of Pharmacy, Fujian Medical University
-
- Chen Jianji
- School of Pharmacy, Fujian Medical University
-
- Wu Zhiqiang
- School of Pharmacy, Fujian Medical University
-
- Wu Guotu
- School of Basic Medical Sciences, Fujian Medical University
-
- Lin Jianzhong
- School of Pharmacy, Fujian Medical University
-
- Chen Yu
- School of Basic Medical Sciences, Fujian Medical University
-
- Xu Yunlu
- School of Pharmacy, Fujian Medical University
この論文をさがす
抄録
Aim: To investigate the thrombolytic and antiplatelet effects of a novel plasminogen activator from the venom of the Gloydius brevicaudus viper (GBV-PA) in vitro and in vivo.<br>Methods: Thrombolytic experiments were performed in rabbit models of ear vein thrombosis and carotid artery thrombosis and in dog model of acute cerebral infarction. Inhibition of thrombus formation was evaluated in rat inferior vena cava thrombosis model and ferric chloride-induced arterial thrombosis. In vitro, we assayed the antithrombotic effect of GBV-PA on rabbit blood clots, euglobulin lysis time (ELT) of rabbit plasma, and ADP-induced platelet aggregation.<br>Results: GBV-PA intravenous administration significantly reduced vascular recanalization times of rabbit ear veins thrombosis and thrombus weight of rabbit carotid artery thrombosis. The arterial recanalization rates were dose- and time-dependently improved after the administration of GBV-PA in canine acute cerebral infarction model. Thrombus length and weight were significantly reduced by GBV-PA both in rat inferior vena cava and ferric chloride-induced arterial thrombosis models. Thrombus formation in the blood of rabbits that were administered of GBV-PA was also inhibited. GBV-PA radically reduced plasma ELT of the rabbit's blood clots. ADP-induced platelet aggregation was inhibited by GBV-PA in a dose-dependent manner with a half-maximal inhibitory concentration of 19.9 μg/mL.<br>Conclusion: This study demonstrates that GBV-PA is a thrombolytic and antiplatelet agent. It has significant antithrombotic effects on various in vitro and in vivo experimental models of thrombosis. The mechanisms that underline its antithrombotic effects were related to GBV-PA's capabilities of increasing fibrinolytic activity and inhibition of platelet aggregation.
収録刊行物
-
- Journal of Atherosclerosis and Thrombosis
-
Journal of Atherosclerosis and Thrombosis 22 (10), 1080-1090, 2015
一般社団法人 日本動脈硬化学会