7.05T MRI を用いたマウス内耳の観察

• 中村 学

  聖マリアンナ医科大学耳鼻咽喉科学教室

• 田中 泰彦

  聖マリアンナ医科大学耳鼻咽喉科学教室

• 肥塚 泉

  聖マリアンナ医科大学耳鼻咽喉科学教室

書誌事項

タイトル別名

• Examination of inner ears of mice using 7.05T MRI

抄録

Endolymphatic hydrops may be involved in the pathogenesis of Meniere's disease. However, the etiology remains to be clarified. Magnetic resonance imaging (MRI) facilitates visualization of the inner ear structure, and is useful for physiological and pharmacological evaluation. Recently, MRI of the fine structures of inner ears has been performed in humans, guinea pigs, and mice. In this study, we conducted high-magnetic-field MRI in mice to visualize endolymphatic hydrops.<br>　An endolymphatic hydrop model, Phex^Hyp-Duk/Y mice, was used, as well as a 7.05T MRI device (AVANCE III, 300 wb, ParaVison 5.1 PL2, Bruker GmbH). After intraperitoneal anesthesia, gadolinium contrast medium diluted with physiological saline at a ratio of 8 was administered into the tympanic cavity.<br>　Under mask anesthesia with sevoflurane, the body temperature was maintained by warm water (38^°C) circulation. A 9-mm surface coil (Doty Co., Ltd.) was established in the external auditory canal. Using the 7.05T MRI device, inner ear imaging was performed. In the Phex^Hyp-Duk/Y mice, the presence of endolymphatic hydrops was confirmed.

収録刊行物

• Ｅｑｕｉｌｉｂｒｉｕｍ　Ｒｅｓｅａｒｃｈ

  Ｅｑｕｉｌｉｂｒｉｕｍ　Ｒｅｓｅａｒｃｈ 74 (4), 308-312, 2015

  一般社団法人 日本めまい平衡医学会

キーワード

• Magnetic resonance imaging
• mouseice
• inner ear

詳細情報

• CRID: 1390282679925212672
• NII論文ID: 130005101946
• DOI: 10.3757/jser.74.308
• ISSN: 1882577X
• ISSN: 03855716
• Web Site: https://www.jstage.jst.go.jp/article/jser/74/4/74_308/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016046636
• 本文言語コード: ja
• データソース種別:
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