Morphological study of boar sperm during their passage through the female genital tract

DOI Web Site Web Site PubMed 被引用文献1件 参考文献48件
  • GARCÍA-VÁZQUEZ Francisco Alberto
    Department of Physiology, Faculty of Veterinary Science, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum”, University of Murcia, Murcia 30100, Spain Institute for Biomedical Research of Murcia (IMIB-Arrixaca), Murcia, Spain
  • HERNÁNDEZ-CARAVACA Iván
    Department of Physiology, Faculty of Veterinary Science, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum”, University of Murcia, Murcia 30100, Spain Boehringer-Ingelheim S.A., Barcelona 08173, Spain
  • MATÁS Carmen
    Department of Physiology, Faculty of Veterinary Science, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum”, University of Murcia, Murcia 30100, Spain Institute for Biomedical Research of Murcia (IMIB-Arrixaca), Murcia, Spain
  • SORIANO-ÚBEDA Cristina
    Department of Physiology, Faculty of Veterinary Science, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum”, University of Murcia, Murcia 30100, Spain Institute for Biomedical Research of Murcia (IMIB-Arrixaca), Murcia, Spain
  • ABRIL-SÁNCHEZ Silvia
    Department of Physiology, Faculty of Veterinary Science, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum”, University of Murcia, Murcia 30100, Spain
  • IZQUIERDO-RICO María José
    Department of Cell Biology and Histology, Faculty of Medicine, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum,” University of Murcia, Murcia 30100, Spain Institute for Biomedical Research of Murcia (IMIB-Arrixaca), Murcia, Spain

この論文をさがす

抄録

Once deposited in the female tract, sperm face a series of challenges that must be overcome to ensure the presence of an adequate normal sperm population close to the site of fertilization. Our aim was to evaluate the influence of the uterine milieu on boar sperm morphology. In experiment 1, sperm morphology was evaluated in the backflow (60 min after insemination) and within the uterotubal junction (UTJ) (collected ~24 h after insemination) following intrauterine sperm deposition (n = 6) and compared with the morphology of the sperm in the insemination dose. In experiment 2, the influence of the uterine fluid (UF) on sperm morphological modifications was evaluated. For this purpose, ejaculated (n = 4) and epididymal (n = 4) sperm were in vitro incubated with or without UF for 2 and 24 h. In both experiments, sperm were classified as normal, having a cytoplasmic droplet (proximal or distal) or having tail defects. The results of experiment 1 pointed to an increase in morphologically abnormal sperm collected in the backflow (27.70%) and a reduction of the same in the UTJ (2.12%) compared with the insemination dose (17.75%) (P < 0.05). In experiment 2, incubation of ejaculated sperm with UF did not provoke any morphological modifications; however, when epididymal sperm were incubated with UF, a pronounced increase in the percentage of normal sperm was evident after 24 h compared with the initial dose (from 25.77% to 53.58%, P < 0.05), mainly due to distal cytoplasmatic droplet shedding (53.22 vs. 20.20%). In conclusion, almost all the sperm that colonize the UTJ had a normal morphology, with part of the abnormal sperm having been discarded in the backflow and part selected/modified on their way to the oviduct. UF seems to influence cytoplasmic distal droplet removal, as demonstrated previously in seminal plasma.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (48)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ