Pathological Examination of Experimentally Induced Periodontal Polyp in Mice

• Matsuda Saeka

  Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry

• Yokoi Yukiko

  Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry

• Moriyama Keita

  Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry

• Shoumura Masahito

  Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry Oral Health Analysis Unit, Matsumoto Dental University Graduate School of Oral Medicine

• Osuga Naoto

  Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry Oral Health Analysis Unit, Matsumoto Dental University Graduate School of Oral Medicine

• Nakano Keisuke

  Department of Oral Pathology and Medicine, Okayama University Graduate School of Medicine, Dentistry and Phamaceutical Sciences

• Kawakami Toshiyuki

  Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine

抄録

The mechanism in the formation of periodontal polyp has been established in several histological studies but details on cell differentiation and/or proliferation have not been elucidated. In the present study, we established a convenient and possible experimental system using ddY mice. Briefly, pentobarbital sodium (Somnopentyl) was injected into the abdominal cavity of the mouse followed by access cavity preparation on maxillary first molar using low speed ½ round bur (Merufa Inc), exposing the pulp and then allowed to perforate the floor of the pulp chamber. Observation was done over time until 6 months using micro CT (m_CT) image photography. Results with transmission image using m_CT showed the expansion in the width of the periodontal ligament in the furcation area. The lesion was excised as one mass and examined histopathologically. The granulation tissue was covered with stratified squamous epithelium. The present experimental technique has been confirmed to be effective in analyzing the formation of periodontal polyp induced by mechanical perforation.

収録刊行物

• Journal of Hard Tissue Biology

  Journal of Hard Tissue Biology 24 (4), 397-400, 2015

  硬組織再生生物学会

キーワード

• Periodontal polyp
• Micro-CT
• Histopathology
• Experimental model

詳細情報

• CRID: 1390282679436258176
• NII論文ID: 130005104332
• NII書誌ID: AA11074332
• DOI: 10.2485/jhtb.24.397
• ISSN: 1880828X
• ISSN: 13417649
• NDL書誌ID: 026831177
• Web Site: https://mdu.repo.nii.ac.jp/records/2556
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026831177
• Web Site: https://www.jstage.jst.go.jp/article/jhtb/24/4/24_397/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016247360
• 本文言語コード: en
• データソース種別:
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  • IRDB
  • NDL
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