Simple and Rapid Immobilization of Coating Polymers on Poly(dimethyl siloxane)-glass Hybrid Microchips by a Vacuum-drying Method

• KITAGAWA Fumihiko

  Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki University

• NAKAGAWARA Syo

  Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki University

• NUKATSUKA Isoshi

  Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki University

• HORI Yusuke

  Department of Material Chemistry, Graduate School of Engineering, Kyoto University

• SUEYOSHI Kenji

  Department of Applied Chemistry, Osaka Prefecture University Graduate School of Engineering

• OTSUKA Koji

  Department of Material Chemistry, Graduate School of Engineering, Kyoto University

抄録

A simple and rapid vacuum-drying modification method was applied to several neutral and charged polymers to obtain coating layers for controlling electroosmotic flow (EOF) and suppressing sample adsorption on poly(dimethyl siloxane) (PDMS)-glass hybrid microchips. In the vacuum-dried poly(vinylpyrrolidone) coating, the electroosmotic mobility (μ_eo) was suppressed from +2.1 to +0.88 × 10⁻⁴ cm²/V·s, and the relative standard deviation (RSD) of μ_eo was improved from 10.2 to 2.5% relative to the bare microchannel. Among several neutral polymers, poly(vinylalcohol) (PVA) and poly(dimethylacrylamide) coatings gave more suppressed and repeatable EOF with RSDs of less than 2.3%. The vacuum-drying method was also applicable to polyanions and polycations to provide accelerated and inversed EOF, respectively, with acceptable RSDs of less than 4.9%. In the microchip electrophoresis (MCE) analysis of bovine serum albumin (BSA) in the vacuum-dried and thermally-treated PVA coating channel, an almost symmetric peak of BSA was obtained, while in the native microchannel a significantly skewed peak was observed. The results demonstrated that the vacuum-dried polymer coatings were effective to control the EOF, and reduced the surface adsorption of proteins in MCE.

収録刊行物

• Analytical Sciences

  Analytical Sciences 31 (11), 1171-1175, 2015

  社団法人 日本分析化学会

キーワード

• Microchip electrophoresis
• vacuum-drying method
• polymer coating
• electroosmotic flow
• bioanalysis

詳細情報

• CRID: 1390282679236161152
• NII論文ID: 130005108598
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.31.1171
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 026859402
• PubMed: 26561262
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026859402
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.31.1171.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.31.1171/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/31/11/31_1171/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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