Regeneration of the Cardiac Conduction System by Adipose Tissue-Derived Stem Cells

• Takahashi Toshinao

  Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine

• Nagai Toshio

  Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine

• Kanda Masato

  Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine

• Liu Mei-Lan

  Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine

• Kondo Naomichi

  Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine

• Naito Atsuhiko T

  Department of Cardiovascular Medicine, The University of Tokyo Hospital

• Ogura Takehiko

  Department of Pharmacology, Chiba University Graduate School of Medicine

• Nakaya Haruaki

  Department of Pharmacology, Chiba University Graduate School of Medicine

• Lee Jong-Kook

  Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine

• Komuro Issei

  Department of Cardiovascular Medicine, The University of Tokyo Hospital

• Kobayashi Yoshio

  Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine

抄録

Background:Adipose tissue is one of the sources of mesenchymal stem cells, which have the potential to differentiate into various types of cells, including myocytes. Whether brown adipose tissue (BAT)-derived cells might differentiate into the cardiac pacemaking-conducting cells, and have the potential to regenerate the cardiac conduction system (CCS), is investigated in this study.Methods and Results:BAT was isolated from the interscapular area of mice and enzymatically digested before culture. Round or fusiform cells showed spontaneous beating at 4–7 days after culturing of BAT-derived cells. Reverse transcriptase-polymerase chain reaction analysis and immunocytochemical analysis revealed that BAT-derived cells expressed several cardiomyocytes, the CCS and pacemaker (PM) cell marker genes and proteins. Patch-clamp techniques revealed that spontaneous electrical activity and the shape of the action potential showed properties of cardiac PM cells. Next, a complete atrioventricular (AV) block was created in mice and green fluorescent protein-positive (GFP (+)) BAT-derived cells were injected intramyocardially around the AV node. At 1 week after transplantation, 50% of BAT-derived cells injected mice showed a sinus rhythm or a 2:1 AV block. Immunohistochemical analysis revealed that injected GFP (+) cells were engrafted and some GFP (+) cells co-expressed several cardiac PM cell marker proteins.Conclusions:BAT-derived cells differentiate into the CCS and PM-like cells in vitro and in vivo, and may become a useful cell source for arrhythmia therapy. (Circ J 2015; 79: 2703–2712)

収録刊行物

• Circulation Journal

  Circulation Journal 79 (12), 2703-2712, 2015

  一般社団法人 日本循環器学会

キーワード

• Brown adipose tissue-derived cells
• Cell transplantation
• Conduction
• Regeneration

詳細情報

• CRID: 1390282680083070080
• NII論文ID: 130005110933
• NII書誌ID: AA11591968
• DOI: 10.1253/circj.cj-15-0400
• ISSN: 13474820
• ISSN: 13469843
• NDL書誌ID: 026944673
• PubMed: 26411528
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026944673
• Web Site: https://www.jstage.jst.go.jp/article/circj/79/12/79_CJ-15-0400/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016232700
• 本文言語コード: en
• データソース種別:
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  • Crossref
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