Overexpression of OCT4A ortholog elevates endogenous XIST in porcine parthenogenic blastocysts
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- HWANG Jae Yeon
- Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea Department of Animal Life Science, Kangwon National University, Chuncheon 200-701, Republic of Korea
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- CHOI Kwang-Hwan
- Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea
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- LEE Dong-Kyung
- Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea
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- KIM Seung-Hun
- Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea
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- KIM Eun Bae
- Department of Animal Life Science, Kangwon National University, Chuncheon 200-701, Republic of Korea
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- HYUN Sang-Hwan
- Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea
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- LEE Chang-Kyu
- Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea Designed Animal & Transplantation Research Institute, Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang 232-916, Republic of Korea
書誌事項
- タイトル別名
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- Overexpression of <i>OCT4A</i> ortholog elevates endogenous <i>XIST</i> in porcine parthenogenic blastocysts
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抄録
X-chromosome inactivation (XCI) is an epigenetic process that equalizes expression of X-borne genes between male and female eutherians. This process is observed in early eutherian embryo development in a species-specific manner. Until recently, various pluripotent factors have been suggested to regulate the process of XCI by repressing XIST expression, which is the master inducer for XCI. Recent insights into the process and its regulation have been restricted in mouse species despite the evolutionary diversity of the process and molecular mechanism among the species. OCT4A is one of the represented pluripotent factors, the gate-keeper for maintaining pluripotency, and an XIST repressor. Therefore, in here, we examined the relation between OCT4A and X-linked genes in porcine preimplantation embryos. Three X-linked genes, XIST, LOC102165544, and RLIM, were selected in present study because their orthologues have been known to regulate XCI in mice. Expression levels of OCT4A were positively correlated with XIST and LOC102165544 in female blastocysts. Furthermore, overexpression of exogenous human OCT4A in cleaved parthenotes generated blastocysts with increased XIST expression levels. However, increased XIST expression was not observed when exogenous OCT4A was obtained from early blastocysts. These results suggest the possibility that OCT4A would be directly or indirectly involved in XIST expression in earlier stage porcine embryos rather than blastocysts.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 61 (6), 533-540, 2015
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206337230976
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- NII論文ID
- 130005113578
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 026989263
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- PubMed
- 26255835
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可