Ultrasound-Assisted Extraction of Pristimerin from Celastrus orbiculatus Using Response Surface Methodology

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  • Fang Gong
    College of Pharmacy, Guangdong Pharmaceutical University
  • Li Guocheng
    Department of Pharmacy, ZengCheng People’s Hospital
  • Pang Chaohai
    College of Pharmacy, Guangdong Pharmaceutical University
  • Li Wenxi
    College of Pharmacy, Guangdong Pharmaceutical University
  • Wang Dingyong
    College of Pharmacy, Guangdong Pharmaceutical University
  • Liu Chunxia
    Department of Pharmacy, ZengCheng People’s Hospital

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  • Ultrasound-Assisted Extraction of Pristimerin from <i>Celastrus orbiculatus</i> Using Response Surface Methodology

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This paper describes the ultrasound-assisted extraction (UAE) of pristimerin from Celastrus orbiculatus. Methanol was the most effective for pristimerin extraction, followed by ethanol, ethyl acetate, n-butanol, and water. To optimize the conditions, the Box–Behnken design, a widely used form of response surface methodology, was used to investigate the effects of parameters on UAE. Several variables, such as extraction time, ultrasonic power, extraction temperature, and solvent-to-solid (S/S) ratio were investigated. The highest extraction yield of 1.843 mg/g was obtained using methanol under optimal conditions with an extraction time of 40 min, ultrasonic power of 105 W, an S/S ratio of 40 mL/g, and an extraction temperature of 52°C. The experimental values under optimal conditions agreed well with the predicted values, suggesting that UAE has good potential as an extraction method for pristimerin from C. orbiculatus.

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