Synthesis of Quaternary Ammonium Derivatives of Cellulose as the Coating Reagents for Capillary Electrophoresis
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- YAMAMOTO Sachio
- Faculty of Pharmaceutical Sciences, Kinki University
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- IWATA Tomoyuki
- Faculty of Pharmaceutical Sciences, Kinki University
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- NISHIWAKI Keiji
- Faculty of Pharmaceutical Sciences, Kinki University
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- KINOSHITA Mitsuhiro
- Faculty of Pharmaceutical Sciences, Kinki University
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- SUZUKI Shigeo
- Faculty of Pharmaceutical Sciences, Kinki University
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In this work, quaternary ammonium group-modified celluloses (QCs) were homogeneously synthesized by reacting cellulose with 3-chloro-2-hydroxypropyltrimethylammonium chloride in NaOH/urea solutions. The structure and solution properties of the QCs were characterized by elemental analysis, FT/IR, 1H-NMR, and size exclusion chromatography. The results show that water-soluble QCs, with degree of substitution values, defined as the substitution of free hydroxyl groups of cellulose, 0.49–0.72 and molecular weight 21–66 kDa could be obtained by optimizing the reaction time. The synthesized QCs were tested for protein separation as physically adsorbed coatings in capillary electrophoresis. Among the derivatives studied, quaternary ammonium cellulose showed rapid electroosmotic mobility and effective suppression of protein adsorption. The EOF can be manipulated for various applications by using QCs with different molecular weight. Because the reversed EOF can be obtained over a broad pH range, it is possible to separate basic, neutral, and acidic proteins under physiological conditions. Eight proteins; lysozyme, ribonuclease A, cytochrome C, α-chymotrypsinogen, α-lactalbumin, ovalbumin, transferrin, and myoglobin were baseline separated within 25 min by 25 mM sodium phosphate buffers (pH 7.0) containing 100 µg/mL QC.
収録刊行物
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- CHROMATOGRAPHY
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CHROMATOGRAPHY 36 (3), 93-98, 2015
クロマトグラフィー科学会
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詳細情報 詳細情報について
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- CRID
- 1390282680722655744
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- NII論文ID
- 130005118200
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- NII書誌ID
- AA1137755X
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- ISSN
- 13483315
- 13428284
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- NDL書誌ID
- 027004171
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可