D-Allose Inhibits Cancer Cell Growth by Reducing GLUT1 Expression
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- Noguchi Chisato
- Department of Cell Physiology, Faculty of Medicine, Kagawa University
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- Kamitori Kazuyo
- Department of Cell Physiology, Faculty of Medicine, Kagawa University
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- Hossain Akram
- Department of Cell Physiology, Faculty of Medicine, Kagawa University
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- Hoshikawa Hiroshi
- Department of Otorhinolaryngology, Faculty of Medicine, Kagawa University
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- Katagi Ayako
- Department of Cell Physiology, Faculty of Medicine, Kagawa University
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- Dong Youyi
- Department of Cell Physiology, Faculty of Medicine, Kagawa University
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- Sui Li
- Department of Cell Physiology, Faculty of Medicine, Kagawa University
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- Tokuda Masaaki
- Department of Cell Physiology, Faculty of Medicine, Kagawa University
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- Yamaguchi Fuminori
- Department of Cell Physiology, Faculty of Medicine, Kagawa University
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抄録
Glucose is a major energy source for mammalian cells and is transported into cells via cell-specific expression of various glucose transporters (GLUTs). Especially, cancer cells require massive amounts of glucose as an energy source for their dysregulated growth and thus over-express GLUTs. d-allose, a C-3 epimer of d-glucose, is one of rare sugars that exist in small quantities in nature. We have shown that d-allose induces the tumor suppressor gene coding for thioredoxin interacting protein (TXNIP) and inhibits cancer cell growth by G1 cell cycle arrest. It has also been reported that GLUTs including GLUT1 are over-expressed in many cancer cell lines, which may contribute to larger glucose utilization. Since d-allose suppresses the growth of cancer cells through the upregulation of TXNIP expression, our present study focused on whether d-allose down-regulates GLUT1 expression via TXNIP expression and thus suppresses cancer cell growth. Western blot and real-time PCR analyses revealed that d-allose significantly induced TXNIP expression and inhibited GLUT1 expression in a dose-dependent manner in three human cancer cell lines: hepatocellular carcinoma (HuH-7), Caucasian breast adenocarcinoma (MDA-MB-231), and neuroblastoma (SH-SY5Y). In these cell lines, d-allose treatment inhibited cell growth. Importantly, d-allose treatment decreased glucose uptake, as measured by the uptake of 2-deoxy d-glucose. Moreover, the reporter assays showed that d-allose decreased the expression of luciferase through the hypoxia response element present in the tested promoter region. These results suggest that d-allose may cause the inhibition of cancer growth by reducing both GLUT1 expression and glucose uptake.
収録刊行物
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 238 (2), 131-141, 2016
東北ジャーナル刊行会