Profiling serum antibodies to Mycobacterium tuberculosis proteins in rhesus monkeys with nontuberculous Mycobacteria

• Min Fangui

  Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, P.R. China Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663, P.R. China

• Pan Jinchun

  Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, P.R. China Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663, P.R. China

• Wu Ruike

  Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, P.R. China Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663, P.R. China

• Chen Meiling

  Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, P.R. China Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663, P.R. China

• Kuang Huiwen

  Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, P.R. China Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663, P.R. China

• Zhao Weibo

  Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, P.R. China Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663, P.R. China

書誌事項

タイトル別名

• Profiling serum antibodies to <i>Mycobacterium tuberculosis</i> proteins in rhesus monkeys with nontuberculous Mycobacteria

抄録

Recent evidence indicates that the prevalence of diseases caused by nontuberculous mycobacteria (NTM) has been increasing in both human and animals. In this study, antibody profiles of NTM in rhesus monkeys (Macaca mulatta) were determined and compared with those of monkeys infected with Mycobacterium tuberculosis complex (MTBC). Antibodies against 10 M. tuberculosis proteins, purified protein derivative (PPD), and mammalian old tuberculin (MOT) were detected in 14 monkeys naturally infected with NTM by indirect ELISA. Sera from 10 monkeys infected with MTBC and 10 healthy monkeys were set as controls. All antigens showed high serological reactivities to MTBC infections and low reactivities in healthy monkeys. NTM infections showed strong antibody responses to MOT and PPD; moderate antibody responses to 16kDa, U1, MPT64L, 14kDa, and TB16.3; and low antibody responses to 38kDa, Ag85b, CFP10, ESAT-6, and CFP10-ESAT-6. According to the criteria of MTBC, only CFP10, ESAT-6, and CFP10-ESAT-6 showed negative antibody responses in all NTM infections. Taken together, these results suggest that positive results of a PPD/MOT-based ELISA in combination with results of antibodies to M. tuberculosis-specific antigens, such as CFP10 and ESAT-6, could discriminate NTM and MTBC infections. Two positive results indicate an MTBC infection, and a negative result for an M. tuberculosis-specific antigen may preliminarily predict an NTM infection.

収録刊行物

• Experimental Animals

  Experimental Animals 65 (1), 11-16, 2016

  公益社団法人 日本実験動物学会

キーワード

• antibody
• <i>M. tuberculosis</i> proteins
• nontuberculous mycobacteria
• rhesus monkeys

詳細情報

• CRID: 1390001205044338304
• NII論文ID: 130005125855
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.15-0027
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 027076708
• PubMed: 26437786
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027076708
• Web Site: https://www.jstage.jst.go.jp/article/expanim/65/1/65_15-0027/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016261935
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可