Screening Method for Novel Rice Starch Mutant Lines Prepared by Introducing Gene Encoding Starch Synthase IIa and Granule-bound Starch Synthase I from Indica Cultivar into a Branching Enzyme IIb-Deficient Mutant Line

• Itoh Yuuki

  Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University

• Crofts Naoko

  Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University

• Abe Misato

  Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University

• F. Oitome Naoko

  Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University

• Fujita Naoko

  Laboratory of Plant Physiology, Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University

抄録

The structure and properties of starch reserves in rice seeds are strongly affected by deficiencies in specific starch biosynthetic enzymes, which are highly expressed in storage tissues. Rice lines with unique seed starches should be utilized for food and industrial applications in the near future. We are currently developing novel rice mutant lines with distinct starch properties by introducing specific genes from different cultivars into mutant lines and by producing multiple combinations of single mutant lines. Obtaining the homozygous genotypes of the target genes is necessary during the screening process of these materials. In this study, we developed an effective, accurate screening method for identifying rice lines with novel starch composition. Specifically, we produced a novel mutant line in which we introduced genes encoding starch synthase IIa (SSIIa) and granule-bound starch synthase I (GBSSI) from indica cultivar into a starch branching enzyme IIb (BEIIb)-deficient mutant line.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ 63 (1), 27-30, 2016

  日本応用糖質科学会

キーワード

• novel starch
• rice mutant
• screening method
• starch branching enzyme
• starch synthase

詳細情報

• CRID: 1390001206293262080
• NII論文ID: 130005126297
• NII書誌ID: AA11809133
• DOI: 10.5458/jag.jag.jag-2015_022
• ISSN: 18807291
• ISSN: 13447882
• NDL書誌ID: 027145367
• Web Site: http://id.ndl.go.jp/bib/027145367
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027145367
• Web Site: https://www.jstage.jst.go.jp/article/jag/63/1/63_jag.JAG-2015_022/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
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  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可