Arterial Vasoreactivity is Equally Affected by <i>In Vivo</i> Cross-Clamping with Increasing Loads in Young and Middle-Aged Mice Aortas
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- Geenens Rachel
- Department of Cardiovascular Sciences, KU Leuven, Herestraat, Leuven, Belgium
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- Famaey Nele
- Department of Mechanical Engineering, KU Leuven, Celestijnenlaan, Heverlee, Belgium
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- Gijbels Andy
- Department of Mechanical Engineering, KU Leuven, Celestijnenlaan, Heverlee, Belgium
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- Verhulst Valérie
- Department of Cardiovascular Sciences, KU Leuven, Herestraat, Leuven, Belgium
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- Vinckier Stefan
- Department of Oncology – Vesalius Research Centre, KU Leuven, Department of Oncology – Vesalius Research Centre, VIB, Herestraat, Leuven, Belgium
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- Vander Sloten Jos
- Department of Mechanical Engineering, KU Leuven, Celestijnenlaan, Heverlee, Belgium
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- Herijgers Paul
- Department of Cardiovascular Sciences, KU Leuven, Herestraat, Leuven, Belgium
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抄録
Purpose: To compensate for the lack of haptic feedback by surgical robots, limitation of exerted forces could be implemented. The limits should be based on the observed relationship between tissue load and induced damage. This study examines whether age-related changes influence this relationship.Methods: Descending thoracic aortas of male C57BL/6J mice of 10, 25 and 40 weeks were clamped in vivo (no clamp, 0.5N or 2.0N) for 2 min. Functional integrity was tested in vitro by studying endothelium-dependent and -independent vasoreactivity.Results: Endothelium-dependent relaxation deteriorated with increased clamping force at all ages. Clamping did not influence endothelium-independent vasodilation. Age (10, 25 and 40 weeks) did not significantly impact on the effect of clamping on endothelium-dependent and independent vasoreactivity.Conclusions: Within the tested conditions, mechanical clamping induces damage to the vascular endothelium, but not to the smooth muscle cells. Age has no effect on the obtained results in mice from 10 to 40 weeks old.
収録刊行物
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- Annals of Thoracic and Cardiovascular Surgery
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Annals of Thoracic and Cardiovascular Surgery 22 (1), 38-43, 2016
Annals of Thoracic and Cardiovascular Surgery 編集委員会