Lipopolysaccharide Delays Closure of the Rat Ductus Arteriosus by Induction of Inducible Nitric Oxide Synthase But Not Prostaglandin E₂

• Kajimura Ichige

  Department of Cell Physiology, The Jikei University School of Medicine

• Akaike Toru

  Department of Cell Physiology, The Jikei University School of Medicine

• Minamisawa Susumu

  Department of Cell Physiology, The Jikei University School of Medicine

書誌事項

タイトル別名

• Lipopolysaccharide Delays Closure of the Rat Ductus Arteriosus by Induction of Inducible Nitric Oxide Synthase But Not Prostaglandin E₂

抄録

Background:The incidence of patent ductus arteriosus is known to be higher in premature neonates with infection than in those without infection. However, the detailed mechanism has not been investigated.Methods and Results:Lipopolysaccharide (LPS; 100 μg/kg) was injected into timed-pregnant Wistar rats on day 18 and 19 of pregnancy. The fetuses were delivered by cesarean section on gestational day 21. Using a rapid whole-body freezing method, it was found that closure of the ductus arteriosus (DA) was significantly delayed in neonates from LPS-injected rats after birth. Histological analysis demonstrated that there was no difference in vascular remodeling of the DA. Quantitative reverse transcriptase-polymerase chain reaction analysis showed that the tumor necrosis factor α and inducible nitric oxide synthase (iNOS) mRNA expression level was significantly increased, but there was no difference in cyclooxygenase 2 and prostaglandin receptor, EP4, mRNA expression in the DA from LPS-injected rats. Moreover, the NOS inhibitor,Nω-Nitro-L-arginine methyl ester hydrochloride, significantly prevented the delayed closure of the DA after birth in neonates from LPS-injected rats.Conclusions:The present study demonstrated that LPS-mediated infection delayed closure of the rat DA without apparent histological changes. iNOS, but not prostaglandin E₂, may play a primary role in delayed functional closure of the DA. (Circ J 2016; 80: 703–711)

収録刊行物

• Circulation Journal

  Circulation Journal 80 (3), 703-711, 2016

  一般社団法人 日本循環器学会

キーワード

• Ductus arteriosus
• Infection
• Lipopolysaccharide
• Nitric oxide

詳細情報

• CRID: 1390001205108757120
• NII論文ID: 130005128466
• NII書誌ID: AA11591968
• DOI: 10.1253/circj.cj-15-1053
• ISSN: 13474820
• ISSN: 13469843
• NDL書誌ID: 027128248
• PubMed: 26821579
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027128248
• Web Site: https://www.jstage.jst.go.jp/article/circj/80/3/80_CJ-15-1053/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016234497
• 本文言語コード: en
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  • Crossref
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