Improvement of the Mutation-Discrimination Threshold for Rare Point Mutations by a Separation-Free Ligase Detection Reaction Assay Based on Fluorescence Resonance Energy Transfer
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- HAGIHARA Kenta
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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- TSUKAGOSHI Kazuhiko
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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- NAKAJIMA Chinami
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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- ESAKI Shinsuke
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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- HASHIMOTO Masahiko
- Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
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We previously developed a separation-free ligase detection reaction assay based on fluorescence resonance energy transfer from a donor quantum dot to an acceptor fluorescent dye. This assay could successfully detect one cancer mutation among 10 wild-type templates. In the current study, the mutation-discrimination threshold was improved by one order of magnitude by replacing the original acceptor dye (Alexa Fluor 647) with another fluorescent dye (Cyanine 5) that was spectrally similar but more fluorescent.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 32 (3), 367-370, 2016
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204259165696
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- NII論文ID
- 130005131641
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 027204061
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- PubMed
- 26960620
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- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I027204061
- https://link.springer.com/content/pdf/10.2116/analsci.32.367.pdf
- https://link.springer.com/article/10.2116/analsci.32.367/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/32/3/32_367/_pdf
- https://search.jamas.or.jp/link/ui/2016313319
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- 本文言語コード
- en
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- NDL
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