Soluble Human Intestinal Lactoferrin Receptor : Ca²⁺-Dependent Binding to Sepharose-Based Matrices
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- Oshima Yuta
- School of Bioscience and Biotechnology, Tokyo University of Technology
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- Seki Kohei
- School of Bioscience and Biotechnology, Tokyo University of Technology
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- Shibuya Masataka
- School of Bioscience and Biotechnology, Tokyo University of Technology
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- Naka Yuki
- School of Bioscience and Biotechnology, Tokyo University of Technology
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- Yokoyama Tatsuya
- School of Bioscience and Biotechnology, Tokyo University of Technology
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- Sato Atsushi
- School of Bioscience and Biotechnology, Tokyo University of Technology
書誌事項
- タイトル別名
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- Soluble Human Intestinal Lactoferrin Receptor: Ca<sup>2+</sup>-Dependent Binding to Sepharose-Based Matrices
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抄録
A soluble form of human intestinal lactoferrin receptor (shLFR) is identical to human intelectin-1 (hITLN-1), a galactofuranose-binding protein that acts as a host defense against invading pathogenic microorganisms. We found that recombinant shLFR, expressed in mammalian cells (CHO DG44, COS-1, and RK13), binds tightly to Sepharose 4 Fast Flow (FF)-based matrices in a Ca2+-dependent manner. This binding of shLFR to Sepharose 4 FF-based matrices was inhibited by excess D-galactose, but not by D-glucose, suggesting that shLFR recognizes repeating units of α-1,6-linked D-galactose in Sepharose 4 FF. Furthermore, shLFR could bind to both Sepharose 4B- and Sepharose 6B-based matrices that were not crosslinked in a similar manner as to Sepharose 4 FF-based matrices. Therefore, shLFR (hITLN-1) binds to Sepharose-based matrices in a Ca2+-dependent manner. This binding property is most likely related to the ability, as host defense lectins, to recognize sepharose (agarobiose)-like structures present on the surface of invading pathogenic microorganisms.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 39 (3), 435-439, 2016
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204632843904
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- NII論文ID
- 130005132305
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 027133903
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- PubMed
- 26934934
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
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- 使用不可