Temperature-Dependent Expression of NodC and Community Structure of Soybean-Nodulating Bradyrhizobia

• Shiro Sokichi

  Faculty of Life and Environmental Science, Shimane University

• Kuranaga Chika

  Faculty of Agriculture, University of Miyazaki

• Yamamoto Akihiro

  Faculty of Agriculture, University of Miyazaki

• Sameshima-Saito Reiko

  Faculty of Agriculture, Shizuoka University

• Saeki Yuichi

  Faculty of Agriculture, University of Miyazaki

書誌事項

タイトル別名

• Temperature-Dependent Expression of <i>NodC</i> and Community Structure of Soybean-Nodulating Bradyrhizobia

抄録

In order to assess the physiological responses of bradyrhizobia and competition for the nodulation of soybean at different temperatures, we investigated the expression of the nodC gene at 20, 25, and 30°C and the abilities of bacteria to nodulate soybean in microcosms at day/night cultivation temperatures of 23/18°C, 28/23°C, and 33/28°C for 16/8 h. We tested five Bradyrhizobium USDA strains: B. diazoefficiens USDA 110^T and 122, B. japonicum USDA 123, and B. elkanii USDA 31 and 76^T. The expression of nodC was up-regulated by increasing culture temperatures in USDA 110^T, 122, 31, and 76^T, but was down-regulated in USDA 123. The proportions of USDA 110^T and 122 within the community were the greatest at 28/23°C. The population of USDA 31 increased, whereas that of USDA 123 decreased with increasing cultivation temperatures. On the other hand, infection by USDA 76^T was not detected, and low numbers of USDA 76^T nodules confirmed its poor nodulation ability. These results indicate that the competitiveness of and infection by USDA 110^T, 122, 123, and 31 for soybean nodulation depend on cultivation temperatures, and suggest that the temperature dependence of nodC expression affects the bradyrhizobial community structure.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 31 (1), 27-32, 2016

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• bradyrhizobia
• <i>nodC</i> gene
• temperature
• community structure

詳細情報

• CRID: 1390282679321489024
• NII論文ID: 130005138793
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me15114
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 027200295
• PubMed: 26877137
• Web Site: http://ir.lib.shimane-u.ac.jp/37164
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027200295
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/31/1/31_ME15114/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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