Utilization of flow cytometry for festulolium breeding (Lolium multiflorum (2x) × Festuca arundinacea (6x))

DOI Web Site Web Site 参考文献37件
  • Akiyama Yukio
    National Research and Development Agency, National Agriculture and Food Research Organization (NARO), Tohoku Agricultural Research Center The United Graduate School of Agricultural Sciences, Iwate University
  • Ueyama Yasufumi
    National Research and Development Agency, National Agriculture and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS)
  • Hamada Seiya
    Department of BioEngineering, Nagaoka University of Technology
  • Kubota Akito
    National Research and Development Agency, National Agriculture and Food Research Organization (NARO), Tohoku Agricultural Research Center
  • Kato Daisuke
    National Research and Development Agency, National Agriculture and Food Research Organization (NARO), Tohoku Agricultural Research Center
  • Yamada-Akiyama Hitomi
    Faculty of Agriculture, Iwate University
  • Takahara Yoshinori
    Department of BioEngineering, Nagaoka University of Technology
  • Fujimori Masahiro
    National Research and Development Agency, National Agriculture and Food Research Organization (NARO), Tohoku Agricultural Research Center The United Graduate School of Agricultural Sciences, Iwate University

書誌事項

タイトル別名
  • Utilization of flow cytometry for festulolium breeding (<i>Lolium multiflorum</i> (2x) × <i>Festuca arundinacea</i> (6x))

この論文をさがす

抄録

Festulolium is a hybrid between Festuca and Lolium species that has valuable agronomic traits from both grass species. The purpose of our breeding program is to produce hexaploid festulolium that introduces tolerance to summer depression into Italian ryegrass (Lolium multiflorum) by crossing it with tall fescue (Festuca arundinacea). However, we found the DNA ploidy of hexaploids was not stable and was reduced in successive generations. We aimed to find out how to obtain stable high-ploidy festulolium. F1 hybrids of L. multiflorum and F. arundinacea were produced. The F3 generation was produced from putative hexaploid F2 individuals by open pollination. The F4 to F6 generations were obtained by polycrossing. The DNA ploidy levels of F2 to F6 individuals were estimated by flow cytometry. Cytological characteristics of the F5 and F6 individuals were investigated by FISH and GISH. The DNA ploidy level of hexaploid festulolium was reduced and stabilized at almost the same level as a tetraploid. Seed fertility was inversely correlated with an increase in ploidy level. GISH revealed no preferential Lolium transmission. FISH with a telomere probe revealed that counting the exact number of chromosomes in festulolium was difficult. DNA ploidy level was strongly correlated with the number of chromosomes.

収録刊行物

参考文献 (37)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ