Time-lapse monitoring reveals that vitrification increases the frequency of contraction during the pre-hatching stage in mouse embryos
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- SHIMODA Yuki
- Department of Obstetrics and Gynecology, Graduate School of Medicine, Akita University, Akita 010-8543, Japan
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- KUMAGAI Jin
- Department of Obstetrics and Gynecology, Graduate School of Medicine, Akita University, Akita 010-8543, Japan
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- ANZAI Mibuki
- Department of Obstetrics and Gynecology, Graduate School of Medicine, Akita University, Akita 010-8543, Japan
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- KABASHIMA Katsuya
- Department of Obstetrics and Gynecology, Graduate School of Medicine, Akita University, Akita 010-8543, Japan
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- TOGASHI Kazue
- Department of Obstetrics and Gynecology, Graduate School of Medicine, Akita University, Akita 010-8543, Japan
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- MIURA Yasuko
- Department of Obstetrics and Gynecology, Graduate School of Medicine, Akita University, Akita 010-8543, Japan
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- SHIRASAWA Hiromitsu
- Department of Obstetrics and Gynecology, Graduate School of Medicine, Akita University, Akita 010-8543, Japan
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- SATO Wataru
- Department of Obstetrics and Gynecology, Graduate School of Medicine, Akita University, Akita 010-8543, Japan
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- KUMAZAWA Yukiyo
- Department of Obstetrics and Gynecology, Graduate School of Medicine, Akita University, Akita 010-8543, Japan
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- TERADA Yukihiro
- Department of Obstetrics and Gynecology, Graduate School of Medicine, Akita University, Akita 010-8543, Japan
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抄録
Contraction during the blastocyst stage is observed during embryonic development of various mammals, including humans, but the physiological role of this process is not well understood. Using time-lapse monitoring (TLM), we studied the influence of vitrification and contractions on embryonic development in mice. Mouse embryos were cultured at the 2-cell stage. At the 8-cell stage, embryos were randomly divided into a fresh group (FG) and vitrified group (VG) and observed for up to 144 h. Strong contractions (i.e., contractions causing a decrease in volume of more than 20% and expansion of the perivitelline space) occurred significantly more often in unhatched embryos than hatching embryos in both groups. Regarding hatching embryos, contractions in the pre-hatching stage were significantly more frequent in the VG than the FG. Furthermore, mRNA expression levels of genes related to contractions were determined at three time points, the 8-cell stage, early blastocyst stage, and 20 h after blastocoel formation, with quantitative reverse transcription-polymerase chain reaction. There was no significant difference in Hspa1a expression between the FG and VG, but Hspa1a overexpression was observed just after thawing and tended to decrease gradually thereafter in some blastocysts. Furthermore, in the VG, Atp1a1 tended to show higher expression in the strong contraction group than in the weak contraction group. Overall, vitrification is an excellent method for cryopreservation but could increase contractions in the pre-hatching stage and may increase energy demands of the embryo. Observation of contraction by TLM may improve the evaluation of embryo quality.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 62 (2), 187-193, 2016
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206337632896
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- NII論文ID
- 130005146468
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 027269952
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- PubMed
- 26806421
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可